中国中药杂志

2004, (11)

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丹参酮微乳逆转肿瘤细胞多药耐药的研究
Effect of tanshinone microemulsion on reversing MDR in human tumor cells

范青,范广俊,杨佩满,赵瑾瑶
FAN Qing1, FAN Guang-jun1, YANG Pei-man2, ZHAO Jin- yao2 (1. Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China; 2. Dalian Medical University, Dalian 116027,China) [

摘要(Abstract):

目的 :研究药物新剂型—丹参酮微乳 (Tan M)在体外对人白血病细胞株K5 6 2的细胞毒作用和对多药耐药 (MDR)的逆转作用。方法 :MTT法检测Tan M对人白血病细胞株K5 6 2 /ADM的细胞毒性和MDR逆转作用 ,设空白微乳 (E M)为对照组。结果 :Tan M非细胞毒性剂量 (生长率≥ 95 % )为 0 2 μg·mL-1;低毒剂量 (生长率 85 %~90 % )为 0 7μg·mL-1。E M无毒剂量相当于 0 7μg·mL-1Tan M同等稀释度 ;低毒剂量相当于 1 2 μg·mL-1Tan M同等稀释度。无毒性剂量的Tan M可显著降低ADM对K5 6 2 /ADM细胞的IC5 0 (P <0 0 1) ,抗药性逆转为 3 88倍 ;低毒剂量的Tan M逆转倍数为 3 97。 0 2 μg·mL-1的E M逆转倍数为 2 6 2。结论 :Tan M体外对人白血病细胞K5 6 2 /ADM有明显的细胞毒作用和MDR逆转作用。
objective: To study the effect of tanshinone mi croemulsion(Tan-M) on the cytotoxicity to human leukemia-cell-line (K562/ ADM) and the reversion of MDR in vitro. Method:Microemulsi o n being supposed as the control group, MTT method is adopted to test cytotoxici ty and the reverse of MDR. Result: Obvious cytotoxicity to K56 2/ADM was observed for tan-M. Cell non-toxic dosage(growth quotiety> 95 %) of Tan-M is 0.2 μg·mL -1 . Low toxic dosage (growth quotiety 85~9 0%)was 0.7 μg·mL -1 . Cell non-toxic dosage of was 0.7 μg·mL -1 and low toxic dosage was 1.2 μg·mL -1 . Cell non-toxic dosage of Tan -M (0.2 μg·mL -1 )significantly lowered the IC 50of K562/A DM by ADM(P<0.01),and reversed MDR was 3.88 times . Low toxic dosage of Tan-M reversed MDR was 3.97 times. E-M (0.2 μg·mL -1 ) reversed MDR was 2.62 times. Conclusion: The result indicates that tanshin one microemulsion possesses cell-toxic effects on human leukemia cell-line an d may reverse MDR of tumor cells. [

关键词(KeyWords): 丹参酮;微乳;多药耐药;逆转作用
tanshinone; microemulison; MDR; reversing func tion

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作者(Author): 范青,范广俊,杨佩满,赵瑾瑶
FAN Qing1, FAN Guang-jun1, YANG Pei-man2, ZHAO Jin- yao2 (1. Second Affiliated Hospital of Dalian Medical University, Dalian 116027,China; 2. Dalian Medical University, Dalian 116027,China) [

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