高姗姗;李建蕊;吴方斌;韦学敏;卞金辉;陈建波;程世琼;孙素琴;吕光华;
GAO Shan-shan;LI Jian-rui;WU Fang-bin;WEI Xue-min;BIAN Jin-hui;CHEN Jian-bo;CHENG Shi-qiong;SUN Su-qin;LV Guang-hua;Key Laboratory of Education Ministry in China for Standardization of Chinese Materia Medica,School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Perkin Elmer Instruments ( Shanghai) Co.,Ltd.;Department of Chemistry,Tsinghua University;Sichuan Provincial Institute for Food and Drug Control;

• 1:成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地
• 2:珀金埃尔默企业管理(上海)有限公司
• 3:清华大学化学系分析中心
• 4:四川省食品药品检验检测院

摘要(Abstract)：

川木通为常用的利尿通淋药;因多来源于野生药材,性状特征差异大,有效成分及特征性成分不明,难以鉴别。为了建立准确鉴别川木通的方法,该研究收集了24份川木通和7份相近中药木通和关木通样品,利用傅里叶变换红外光谱技术测定4 000~400 cm-1的一维和二维相关红外光谱。首先随机选取20份川木通样品为校正集样品,发现其红外光谱的峰形、峰位、峰强相似,相关系数大于0.994 3;以其模拟平均红外光谱为指纹图谱,其特征峰位于3 412,2 932,1 739,1 639,1 509,1 456,1 426,1 376,1 332,1 261,1 159,1 035,897,609 cm-1等。然后,根据统计学的"3σ规则",计算用红外光谱相关系数判别川木通真伪的域值(Mean-3σ=0.992 6)。再用另外4份川木通样品、4份关木通和3份木通样品检验该指纹图谱鉴别川木通的效果。结果表明,这4份川木通的红外光谱均与指纹图谱相似,相关系数也大于域值而判别为川木通;而关木通和木通与川木通的指纹图谱明显不同,相关系数均小于域值而判别为非川木通。进一步比较了川木通、木通、关木通的二维相关红外光谱,发现自动峰和交叉峰数目、位置、强度等特征均不同。由此可见,建立的川木通红外光谱指纹图谱可用于川木通的真伪鉴别,为川木通提供了一种简便、快速鉴别的新方法。
Armand clematis stem( Clematidis Armandii Caulis,Chuanmutong) is a widely used Chinese herb to disinhibit urine and relieve stranguria. It is difficult to be identified owing to its various macroscopic feature and unknown characteristic compounds.Thus,total of 24 Chuanmutong samples and 7 related herbs including four manshurian aristolochia stem( Aristolochiae Manshuriensis Caulis,Guanmutong) and three akebia stem( Akebiae Caulis,Mutong) samples were collected and analyzed in the range of 4 000-400 cm-1by Fourier Transform Infrared( FTIR) and two-dimensional infrared correlation spectroscopy( 2D-FTIR) techniques. The FTIR spectra of 24 Chuanmutong samples are consistent in the spectrum profiles,position and intensity of characteristic peaks. 20 of the 24 Chuanmutong samples were randomly selected as calibration samples to calculate and simulate mean spectrum. This mean spectrum is named as FTIR fingerprint of Chuanmutong with characteristic peaks at 3 412,2 932,1 739,1 639,1 509,1 456,1 426,1 376,1 332,1 261,1 159,1 035,897,609 cm-1. Meanwhile,the limited level( Mean-3σ = 0. 992 6) to identify true or false Chuanmutong by correlation coefficient of FTIR spectra was calculated based on the 20 Chuanmutong calibration samples. Then,the rest 4 Chuanmutong,4 Guanmutong and 3 Mutong samples were used as validation samples to evaluate the identification efficacy. The result shows that the FTIR spectra of 4 Chuanmutong validation samples were similar to the fingerprint. Their correlation coefficients of FTIR spectra were over the limited level and accepted as Chuanmutong. However,the spectra of Guanmutong and Mutong were significantly different from Chuanmutong fingerprint. The correlation coefficients of Guanmutong( 0. 902 1-0. 940 4,n = 4) and Mutong( 0. 954 9-0. 978 9,n = 3) FTIR spectra were less than the limited level and rejected from Chuanmutong. Furthermore,the number,position and intensity of auto-peaks on the 2D-FTIR were drastically different among the three herbs. It is concluded that the developed FTIR fingerprinting can be rapidly and accurately identify Chuanmutong and differentiate from related herbs.

关键词(KeyWords)： 川木通;关木通;木通;红外光谱;指纹图谱;相关系数;鉴别
Clematidis Armandii Caulis;manshurian aristolochia stem;akebia stem;Fourier transform infrared;fingerprint;correlation coefficient;identification

Abstract:

Keywords:

基金项目(Foundation): 国家基础科学人才培养基金项目(J1310034-02);; 四川省科技计划项目(2014SZ0071)

作者(Author): 高姗姗;李建蕊;吴方斌;韦学敏;卞金辉;陈建波;程世琼;孙素琴;吕光华;
GAO Shan-shan;LI Jian-rui;WU Fang-bin;WEI Xue-min;BIAN Jin-hui;CHEN Jian-bo;CHENG Shi-qiong;SUN Su-qin;LV Guang-hua;Key Laboratory of Education Ministry in China for Standardization of Chinese Materia Medica,School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Perkin Elmer Instruments ( Shanghai) Co.,Ltd.;Department of Chemistry,Tsinghua University;Sichuan Provincial Institute for Food and Drug Control;

Email:

DOI:

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