中国中药杂志

2004, (04)

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喇叭唇石斛组织培养的研究
Studies on tissue culture of Dendrobium lituiflorum

常俊,丁小余,保曙琳,刘冬扬,贺佳,唐凤,丁秉中
CHANG Jun, DING Xiao yu, BAO Shu ling,LIU Dong yang, HE Jia, TANG Feng, DING Bing zhong (School of Life Science, Nanjing University, Nanjing Normal 210097, China)

摘要(Abstract):

目的 :筛选喇叭唇石斛快速繁殖体系的适宜培养基及培养条件。方法 :对基本培养基、光照条件、激素、天然提取物浓度等进行比较研究。进行叶绿素含量、可溶性蛋白含量测定。结果 :N6培养基是种胚萌发成苗适宜培养基 ,而 1/ 2MS +NAA 0 .5mg·L-1易于原球茎膨大增殖。先暗培养 2 0d再光照培养有利于胚的萌发及苗的生长。 1/ 2MS +NAA 0~ 0 .5mg·L-1有利于原球茎大量增殖 ,N6+6 BA 2 .0mg·L-1+NAA 0 .5mg·L-1+10 %马铃薯汁是原球茎分化的适宜培养基 ,添加激素和马铃薯汁促进叶绿素和可溶性蛋白含量上升。N6+NAA 0 .5mg·L-1+10 %香蕉汁生根壮苗最适宜。结论 :无机盐浓度、氮源形态、光照条件对胚萌发生长影响较大 ;氮源形态对原球茎增殖有影响 ;叶绿素含量、可溶性蛋白含量能反映组培苗的生长状态 ,可作为筛选培养基参考指标
Objective: To select optimal media and conditions for the tissue culture of Dendrobium lituiflorum. Method: The basic media, light illumination condition, phytohormone, and natural aqueous extracts. were tested and compared. The chlorophyll contents and souble protein contents were measured. Result: N 6 medium was suitable for embryo germination and growth. 1/2 MS+NAA 0.5 mg·L -1 cordd be used as the optimal protocorm multiplying media. Culture 20 days in darkness in advance, and then in light is useful to embryo germinate. 1/2 MS+ NAA 0~0.5 mg·L -1 is beneficial to protocorm multiplication largely. N 6+6 BA 2.0 mg·L -1 +NAA 0.5 mg·L -1 +10%potato juice is useful to protocorm differentiation. Phytohormone and potato juice added to the media promoted chlorophyll content and souble protein content. N 6+NAA 0.5 mg·L -1 +10%banana juice is the best medium for plantlet rootage and strengthening. Conclusion: Concentration of inorganic salt, nitrogen source and illumination condition are important to embryo germination and growth. The nitrogen source type has effect on the protocorm multiplication. The chlorophyll contents and souble protein contents may be index to indicate the growth condition of plantlets, which can help to select the optimal media.

关键词(KeyWords): 喇叭唇石斛;组织培养
Dendrobium lituiflorum; tissue culture

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作者(Author): 常俊,丁小余,保曙琳,刘冬扬,贺佳,唐凤,丁秉中
CHANG Jun, DING Xiao yu, BAO Shu ling,LIU Dong yang, HE Jia, TANG Feng, DING Bing zhong (School of Life Science, Nanjing University, Nanjing Normal 210097, China)

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