中国中药杂志

2010, v.35(21) 2829-2833

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附片指纹图谱研究及6种酯型生物碱含量测定
Study on HPLC fingerprinting and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice

陈东安;易进海;黄志芳;刘云华;吴燕;李晓亮;
CHEN Dong′an1,2,YI Jinhai1,HUANG Zhifang1,LIU Yunhua1,WU Yan2,LI Xiaoliang3(1.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;2.Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;3.Luzhou Medical College,Luzhou 646000,China)

摘要(Abstract):

目的:采用高效液相色谱法建立白附片、黑附片的HPLC指纹图谱,并同时测定6种酯型生物碱的含量,为附子炮制和附片安全使用提供科学依据。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm,5μm),乙腈-0.04 mol.L-1醋酸铵溶液(浓氨溶液调pH10.0)梯度洗脱。用国家药典委员会"相似度评价软件2004AB"处理分析,建立白附片、黑附片的HPLC指纹图谱,并测定白附片、黑附片、黄附片、生附子和盐附子中6种酯型生物碱的含量。结果:各批次白附片、黑附片指纹图谱相似度高,6种酯型生物碱分离度良好,黄附片、生附子指纹图谱与白附片、黑附片的对照图谱显著不同。结论:本研究所建立的方法可靠、简便易行。附片指纹图谱专属性强,结合6种酯型生物碱含量能较全面地反映其内在质量,为附片的安全使用及其质量控制提供科学依据。
Objective: To establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.Method: HPLC analysis was performed on a Phenomenex Gemini C18(4.6 mm×250 mm,5 μm)with 0.04 mol·L-1 ammonium acetate(adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004 AB)was used in data analysis.Result: Baifupian,Heifupian from different samples were of high similarity in fingerprint,and the separation of six kinds of ester-type alkaloids was good.Huangfupian and crude aconite root showed significant difference in fingerprint,comparing with Baifupian and Heifupian.Conclusion: This method is simple and reliable.The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality contro1.

关键词(KeyWords): 附片;HPLC指纹图谱;6种酯型生物碱;含量测定
Radix Aconiti lateralis praeparata slice;HPLC fingerprint;six kinds of ester-type alkaloids;determination.

Abstract:

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基金项目(Foundation): 国家重点基础研究发展计划项目(973计划)(2009CB522804)

作者(Author): 陈东安;易进海;黄志芳;刘云华;吴燕;李晓亮;
CHEN Dong′an1,2,YI Jinhai1,HUANG Zhifang1,LIU Yunhua1,WU Yan2,LI Xiaoliang3(1.Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China;2.Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;3.Luzhou Medical College,Luzhou 646000,China)

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