中国中药杂志

2020, v.45(12) 2947-2953

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四物汤治疗原发性痛经的方剂配伍内涵及作用机制研究
Prescription compatibility connotation and action mechanism of Siwu Decoction in treating primary dysmenorrhea

李杰;江华娟;何瑶;石金凤;陈意;罗尧尧;傅超美;
LI Jie;JIANG Hua-juan;HE Yao;SHI Jin-feng;CHEN Yi;LUO Yao-yao;FU Chao-mei;College of Pharmacy, Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

探究四物汤(SWD)治疗原发性痛经(PD)的方剂配伍科学内涵并验证经网络药理学法预测的SWD治疗PD的机制。联用苯甲酸雌二醇-缩宫素构建小鼠PD模型;PD小鼠随机分为正常组、模型组、阳性组、全方组、缺熟地黄组、缺白芍组、缺挥发油组、缺川芎-当归组,以其潜伏期、扭体次数、扭体抑制率、血清中前列腺素F_(2α)(PGF_(2α))和前列腺素(PGE_2)含量、子宫匀浆中内皮素-1和Ca~(2+)含量以及前列腺素G/H合酶2(PTGS2)、雌激素受体(ESR1)、糖皮质激素受体(NR3C1)mRNA和蛋白表达水平与子宫组织病理变化为指标探究SWD治疗PD的方剂配伍科学内涵并验证其网络预测作用机制。与全方提取液相比,含有挥发油的缺熟地黄组和缺白芍组效果较全方提取液略有降低,缺挥发油组效果显著降低,缺川芎、当归药材提取液组效果最差。SWD能显著降低小鼠PTGS2蛋白和mRNA相对表达量;显著升高ESR1,NR3C1蛋白和mRNA相对表达量。SWD通过调节关键蛋白PTGS2,ESR1,NR3C1的表达治疗PD,其主要药味是当归、川芎,有效成分主要存在于挥发油中,但白芍、熟地黄亦有一定贡献。
The aim of this paper was to study the prescription compatibility connotation in the treatment of primary dysmenorrhea(PD) and verify the mechanism as predicted by network pharmacology of Siwu Decoction(SWD). Mice PD model was constructed by using estradiol benzoate-oxytocin. PD mice were randomly divided into 8 groups, namely normal group, model group, positive group, complete formula group, Rehmanniae Radix Praeparata-free group, Paeoniae Radix Alba-free group, volatile oil-free group, Chuan-xiong Rhizoma and Angelicae Sinensis Radix-free group. Latent time, writhing times, inhibition rate, prostaglandin F_2_α(PGF_2_α) and prostaglandin E_2(PGE_2) levels in serum, endothelin-1, Ca~(2+), expression levels of prostaglandin synthase 2 G/H(PTGS2), estrogen receptor(ESR1), glucocorticoid receptor gene(NR3 C1) mRNA and protein expression levels in the uterus homogenate and pathological changes of uterine tissue were index to explore the prescription compatibility connotation and verify the mechanism of SWD in the treatment of PD. Compared with the extraction liquid of the whole recipe, the effect of Rehmanniae Radix Praeparata-free group and Paeoniae Radix Alba-free group with volatile oil were slightly lower, the effect of essential oil-free group was significantly lower, and the effect of Chuanxiong Rhizoma and Angelicae Sinensis Radix-free group was worse than that of the whole recipe. The relative expression levels of PTGS2 protein and mRNA were significantly reduced by the SWD. The relative expressions of protein and mRNA of ESR1, NR3 C1 were significantly increased. SWD treats PD by regulating the expression of key proteins PTGS2, ESR1 and NR3 C1.Its main medicinal herbs were Angelicae Sinensis Radix and Chuanxiong Rhizoma. Active components were mainly in volatile oil, but Paeo-niae Radix Alba and Rehmanniae Radix Praeparata also had some contributions.

关键词(KeyWords): 四物汤;原发性痛经;作用机制,关键蛋白;mRNA
Siwu Decoction;primary dysmenorrhea;action mechanism;key proteins;mRNA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81303238);; 四川省重点研发项目(18ZDYF3528)

作者(Author): 李杰;江华娟;何瑶;石金凤;陈意;罗尧尧;傅超美;
LI Jie;JIANG Hua-juan;HE Yao;SHI Jin-feng;CHEN Yi;LUO Yao-yao;FU Chao-mei;College of Pharmacy, Chengdu University of Traditional Chinese Medicine;

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