中国中药杂志

2015, v.40(09) 1776-1782

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裸花紫珠指纹图谱研究及10种成分的含量测定
Study on HPLC fingerprint of Callicarpa nudiflora and determination of ten components

郑东昆;陈伟康;马双成;王杰;罗晨煖;罗跃华;
ZHENG Dong-kun;CHEN Wei-kang;MA Shuang-cheng;WANG Jie;LUO Chen-xuan;LUO Yue-hua;Jiangxi Provincial Research Institute for Drug Control;School of Pharmacy,Nanchang University;National Institutes for Food and Drug Control;

摘要(Abstract):

建立裸花紫珠叶的HPLC指纹图谱,并同时测定其中10种成分的含量,为其质量控制及鉴定提供科学依据。采用Waters Sunfire C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.1%甲酸(B),梯度洗脱,流速为1 m L·min-1,检测波长330 nm,柱温30℃。运用中药色谱指纹图谱相似度评价系统(2012)进行分析。各批次裸花紫珠叶的指纹图谱相似度较高,10种成分分离度良好。裸花紫珠枝及同属4种紫珠与裸花紫珠叶对照图谱存在明显差异,定量分析条件通过方法学验证,平均加样回收率在96.0%~105.0%。所建立的定性、定量分析方法简便,准确度高,专属性强,可作为裸花紫珠鉴定及质量控制的依据。
This study is to establish the HPLC fingerprint and determine eight components of Callicarpa nudiflora,and provide a scientific basis for the identification and quality control. The Waters sunfire C18column( 4. 6 mm × 250 mm,5 μm) was used and the detection wavelength was 330 nm. The column temperature was 30 ℃. The mobile phases were acetonitrile( A) and 0. 1% formic acid( B) eluting in a gradient program at a flow rate of 1. 0 m L·min- 1. The chromatographic fingerprint similarity evaluation system for tradition Chinese medicine( 2012) was used for analysis. C. nudiflora from different samples were of high similarity in fingerprint and the separation of ten components was good. There was an obvious difference between other samples and C. nudiflora leaves. In quantitative analysis,the ten components showed good regression( R2> 0. 999 0) with linear ranges,and their recoveries were in the range of96. 0%-105. 0%. The established qualitative and quantitative methods are highly specific,simple and accurate,which can be used for the identification and quality control of C. nudiflora.

关键词(KeyWords): 裸花紫珠叶;HPLC;指纹图谱;含量测定
Callicarpa nudiflora leaves;HPLC;fingerprint;determination

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基金项目(Foundation): 国家自然科学基金项目(81373955);; 中国食品药品检定研究院中青年发展研究基金课题项目(2013WA9);; 江西省自然科学基金项目(20142BAB205085)

作者(Author): 郑东昆;陈伟康;马双成;王杰;罗晨煖;罗跃华;
ZHENG Dong-kun;CHEN Wei-kang;MA Shuang-cheng;WANG Jie;LUO Chen-xuan;LUO Yue-hua;Jiangxi Provincial Research Institute for Drug Control;School of Pharmacy,Nanchang University;National Institutes for Food and Drug Control;

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