中国中药杂志

2018, v.43(16) 3322-3328

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指纹图谱、模式识别结合一测多评法在广金钱草质量评价中的应用研究
Quality evaluation of Desmodium styracifolium by fingerprint,pattern recognition combined with quantitative analysis of multi-components by single marker

陈亮元;程轩轩;唐晓敏;杨全;
CHEN Liang-yuan;CHENG Xuan-xuan;TANG Xiao-min;YANG Quan;Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials,Guangzhou Comprehensive Experimental Station of National Industrial Technology System for Chinese Materia Medica,Guangdong Engineering Research Center of Good Agricultural Practice & Comprehensive Development for Cantonese Medicinal Materials,School of Traditional Chinese Medicine,Guangdong Pharmaceutical Univ

摘要(Abstract):

采用Phenomenex PS C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%甲酸水溶液(30∶70),体积流量0.8 mL·min~(-1),柱温30℃,检测波长335 nm,进样量10μL,建立了不同产地广金钱草Desmodium styracifolium的HPLC指纹图谱,确定了10个共有峰,鉴定了其中5个共有峰:维采宁-1、夏佛塔苷、异荭草苷、异夏佛塔苷、异牡荆苷。通过相似度评价、聚类分析、主成分分析和偏最小二乘判别分析方法对21批广金钱草药材指纹图谱进行研究,进而建立了一测多评法同时测定广金钱草药材中上述5种黄酮苷成分,为广金钱草的质量控制和品质评价提供参考。
HPLC analysis was performed on a Phenomenex PS C_(18)( 4. 6 mm × 250 mm,5 μm) column using methanol-0. 2% formic acid( 30∶ 70) at a flow rate of 0. 8 mL·min~(-1). The column temperature was 30 ℃ and the detection wavelength was set at 335 nm. The injection volume was 10 μL. The HPLC fingerprint of Desmodium styracifolium was established with 10 common peaks,and 5 of them were identified as vicenin~(-1),schaftoside,isoorientin,isoschaftoside and isovitexin,respecivetly. The fingerprints of 21 batches of D. styracifolium samples were analyzed with similarity evaluation,cluster analysis,principal component analysis and partial least squares discriminant analysis. There was no significant difference among the quantitative results of these five ingredients verified by external standard method( ESM) and quantitative analysis of multi-components by single marker( QAMS) method. The application of fingerprint,pattern recognition combined with QAMS can provide more comprehensive references for the quality control and evaluation of D. styracifolium.

关键词(KeyWords): 广金钱草;HPLC;指纹图谱;模式识别;一测多评法
Desmodium styracifolium;HPLC;fingerprint;pattern recognition;quantitative analysis of multi-components by single marker

Abstract:

Keywords:

基金项目(Foundation): 国家新兴产业重大工程包中药标准化项目(ZYBZ-Y-GD-13)

作者(Author): 陈亮元;程轩轩;唐晓敏;杨全;
CHEN Liang-yuan;CHENG Xuan-xuan;TANG Xiao-min;YANG Quan;Key Laboratory of State Administration of Traditional Chinese Medicine for Production & Development of Cantonese Medicinal Materials,Guangzhou Comprehensive Experimental Station of National Industrial Technology System for Chinese Materia Medica,Guangdong Engineering Research Center of Good Agricultural Practice & Comprehensive Development for Cantonese Medicinal Materials,School of Traditional Chinese Medicine,Guangdong Pharmaceutical Univ

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DOI: 10.19540/j.cnki.cjcmm.2018.0097

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