中国中药杂志

2020, v.45(19) 4658-4666

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

利用HPLC-IT-TOF-MS分析新疆一枝蒿的化学成分
Chemical profiling of Artemisia rupestris using HPLC-IT-TOF-MS

张佩杰;曹妍;张珂;宋月林;李军;唐丽;
ZHANG Pei-jie;CAO Yan;ZHANG Ke;SONG Yue-lin;LI Jun;TANG Li;Key Laboratory of Ethnomedicine (Minzu University of China), Ministory of Education, School of Pharmacy, Minzu University of China;Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine;

摘要(Abstract):

新疆一枝蒿是维吾尔族、哈萨克族的传统常用药,具有活血解毒、去积滞、清热消食等功效。目前,新疆一枝蒿的化学成分尚未被深入阐明,很大程度上制约了该药体内药效物质的系统阐明和质量标准的制定。该文利用HPLC-IT-TOF-MS对新疆一枝蒿中的化学成分进行定性分析,从新疆一枝蒿75%乙醇提取物中共检测到124个化合物,通过与对照品比对,准确鉴定了7个绿原酸类、4个黄酮类和1个倍半萜类化合物;通过匹配多级质谱信息与文献数据以及质谱裂解规律的推导,初步鉴定了20个倍半萜类、65个黄酮类和23个绿原酸类化合物。该文对新疆一枝蒿的化学成分进行全面解析,为新疆一枝蒿的体内药效物质及质量标准的建立提供了有效信息。
Artemisia rupestris is widely used as a folk medicine of Uygur and Kazak with blood and detoxification, deaccumulation stagnation, clearing heat digestion and other effects. Currently, the chemical profile of A. rupestris has not been disclosed, resulting in a great obstacle for the systematic clarification of the efficacy materials and the quality evaluations. In this paper, HPLC-IT-TOF-MS was deployed to characterize the chemical constituents in A. rupestris. As a result, a total of 124 compounds were detected in 75% ethanol extract of A. rupestris. By comparing with the reference compounds, seven chlorogenic acids, and four flavonoids as well as one sesquiterpenoid were definitely identified. Moreover, twenty sesquiterpenes, sixty-five flavonoids and twenty-three chlorogenic acids were preliminarily identified by matching MS/MS spectral information with literature data and applying those empirical mass spectrometric cracking rules. In current study, the chemical composition of A. rupestris was profiled in depth, and the findings are envisioned to provide a theoretical basis for the further studies of this well-known herbal medicine, such as efficacy material characterization and quality assessment.

关键词(KeyWords): 新疆一枝蒿;倍半萜类;黄酮;绿原酸;质谱裂解规律;HPLC-IT-TOF-MS
Artemisia rupestris;sesquiterpenoids;flavone;chlorogenic acid;mass fragmentation pathways;HPLC-IT-TOF-MS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81873397);; 民族医药教育部重点实验室课题(KLEM-ZZ201803)

作者(Author): 张佩杰;曹妍;张珂;宋月林;李军;唐丽;
ZHANG Pei-jie;CAO Yan;ZHANG Ke;SONG Yue-lin;LI Jun;TANG Li;Key Laboratory of Ethnomedicine (Minzu University of China), Ministory of Education, School of Pharmacy, Minzu University of China;Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica,Beijing University of Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20200416.202

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享