中国中药杂志

1997, (04)

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香港市售蒲公英及其混淆品土公英的DNA指纹鉴别研究
Identification of Herba Taraxaci and Its Adulterants in Hong Kong Market by DNA Fingerprinting with Random Primed PCR

曹晖,毕培曦,邵鹏柱
Cao Hui, Paul Puihay But and Shaw Pangtchui (Departments of Biology and Biochemistry and Chinese Medicinal Material Research Centre, The Chinese University of Hong Kong)

摘要(Abstract):

采用分子生物技术包括任意引物聚合酶链式反应(APPCR)和随意引物扩增的多态性DNA(RAPD)方法扩增蒲公英及其6种土公英混淆品的基因组DNA,获得清晰可靠的DNA指纹图谱。根据琼脂糖凝胶上显示的DNA带型差异可鉴别蒲公英和土公英混淆品。
DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random primed polymerase chain reaction (PCR) including arbitrarily primed PCR(AP PCR) and random amplified polymorphic DNA(RAPD).Distinctive genomic fingerprints from DNA of Taraxacum mongolicum and its adulterants were generated with two long (20 24 mer )and one short (10 mer) random chosen primers Taraxacum mongolicum can be distinguished from six adulterants according to the banding patterns of their amplified DNA on agarose gels. Moreover,that Taraxacum mongolicum and its adulterant are unrelated. The Similarity Indexes values of the genomic fingerprints.Results showed AP PCR and RAPD might be used for identifying Chinese drugs.

关键词(KeyWords): 药材鉴定,蒲公英,聚合酶链式反应,DNA指纹图谱
Chinese drug;identification; Taraxacum mongolicum; polymerase chain reaction; DNA fingerprinting

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基金项目(Foundation): 香港政府研究资助局资助

作者(Author): 曹晖,毕培曦,邵鹏柱
Cao Hui, Paul Puihay But and Shaw Pangtchui (Departments of Biology and Biochemistry and Chinese Medicinal Material Research Centre, The Chinese University of Hong Kong)

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