康传志;蒋靖怡;杨婉珍;周利;吕朝耕;李佳兴;王升;周涛;杨野;黄璐琦;郭兰萍;
KANG Chuan-zhi;JIANG Jing-yi;YANG Wan-zhen;ZHOU Li;LV Chao-geng;LI Jia-xing;WANG Sheng;ZHOU Tao;YANG Ye;HUANG Lu-qi;GUO Lan-ping;State Key Laboratory and Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Guiyang College of Traditional Chinese Medicine;Kunming University of Science and Technology;

• 1:中国中医科学院中药资源中心道地药材国家重点实验室培育基地
• 2:贵阳中医学院
• 3:昆明理工大学

摘要(Abstract)：

特征硫熏标志物研究已成为中药质量控制的有效辅助手段。该研究建立了UPLC-MS/MS测定天麻硫熏产物p-hydroxybenzyl hydrogen sulfite的定量分析方法。应用所建立的方法对37批天麻药材进行筛查,发现27批样品是经过硫熏处理的,硫熏较严重的为云南产区的天麻样品。通过考察不同贮藏时间和加热处理下p-hydroxybenzyl hydrogen sulfite成分的稳定性,发现药材中该成分含量在8个月的贮藏期内以及正常加热处理(50,60,70,80℃)条件下是稳定可靠的。以上研究表明该硫熏产物可作为天麻硫熏标志物,具有灵敏度高、选择性好和稳定性好,为天麻质量控制和硫熏药材筛查提供参考。
Studies on the characteristic chemical markers of sulfur fumigation have become an effective auxiliary way for quality control of traditional Chinese medicine. This study established a quantitative analysis method for the determination of p-hydroxybenzyl hydrogen sulfite( p-HS) in Gastrodiae Rhizoma( GR) based on UPLC-MS/MS. Then,37 batches of GR were screened,and the results showed that 27 batches of them were sulfur-fumigated. Especially,the GR samples in Yunnan producing areas were sulfur-fumigated more seriously. Based on the stability of p-HS after different storage time and heat treatment methods,it was found that the content of p-HS was stable and reliable in the storage period of 8 months and under normal heat treatment( 50,60,70,80 ℃) conditions. In conclusion,this study shows a high sensitivity,good selectivity and good stability of p-HS,which can provide reference for the quality control and sulfur fumigation screening of GR

关键词(KeyWords)： 天麻;硫熏标志物;UPLC-MS/MS;稳定性;质量评价
Gastrodiae Rhizoma;chemical marker of sulfur fumigation;UPLC-MS/MS;stability;quality evaluation

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81325023,81473307);; 国家重点研发计划项目(2017YFC1700701);; 中央本级重大增减支项目(2060302);; 发改委标准化项目(ZYBZH-C-HLJ-17);; 中国中医科学院博士研究生创新人才培养基金项目(CX201705);; 云南省科技项目(2017 ZF014)

作者(Author): 康传志;蒋靖怡;杨婉珍;周利;吕朝耕;李佳兴;王升;周涛;杨野;黄璐琦;郭兰萍;
KANG Chuan-zhi;JIANG Jing-yi;YANG Wan-zhen;ZHOU Li;LV Chao-geng;LI Jia-xing;WANG Sheng;ZHOU Tao;YANG Ye;HUANG Lu-qi;GUO Lan-ping;State Key Laboratory and Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Guiyang College of Traditional Chinese Medicine;Kunming University of Science and Technology;

Email:

DOI: 10.19540/j.cnki.cjcmm.20171023.003

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