沈岚;林晓;洪燕龙;梁爽;袁莹;冯怡;徐德生;阮克锋;
SHEN Lan1,2,LIN Xiao1,2,HONG Yanlong1,LIANG Shuang1,YUAN Ying1,FENG Yi1,XU Desheng3,RUAN Kefeng1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.College of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai

• 1:上海中医药大学中药现代制剂技术教育部工程研究中心
• 2:上海中医药大学中药学院
• 3:上海中医药大学附属曙光医院

摘要(Abstract)：

目的:通过大川芎方效应组分血浆及脑脊液HPLC指纹图谱的研究,建立准确、简便的体内定性分析方法,为大川芎方入血、入脑成分的研究奠定基础,对阐明其"引药上行"配伍规律提供依据。方法:建立大川芎方效应组分血浆及脑脊液HPLC指纹图谱。结果:各共有峰的相对保留时间的RSD<2.0%,6批血浆、脑脊液样品的指纹图谱的相似度均在0.85以上。结论:所建立的大川芎方效应组分血浆、脑脊液指纹图谱相对稳定、准确,可用于体内成分的定性分析。
Objective: To establish an accurate and simple qualitative analytical method in vivo in the study on HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid,in order to lay a foundation for studies on components found in plasma and cerebrospinal fluid and provide basis for compatible regularity of initiators.Method: HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid was established.Result: The similarity of fingerprints of all of six batches of samples was above 0.85,RSD of their relative retention times in common peaks was less than 2.0%.Conclusion: The fingerprints of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid were stable and accurate that they can be used in qualitative analysis on in vivo components.

关键词(KeyWords)： 大川芎方效应组分;指纹图谱;血浆;脑脊液
active component of Dachuanxiong Fang;fingerprint;plasma;cerebrospinal fluid

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(30801548);; 国家“重大新药创制”科技重大专项(2009ZX09502-009);; 国家教育部科学技术研究重点项目(211060);; 上海市教育委员会科研创新项目(12ZZ124,11ZZ111);上海市教委重点学科项目(J50302);; 上海市自然基金项目(11ZR1434500)

作者(Author): 沈岚;林晓;洪燕龙;梁爽;袁莹;冯怡;徐德生;阮克锋;
SHEN Lan1,2,LIN Xiao1,2,HONG Yanlong1,LIANG Shuang1,YUAN Ying1,FENG Yi1,XU Desheng3,RUAN Kefeng1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.College of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai

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参考文献(References)：

• [1]刘林,方永奇.醒脑开窍中药对血脑屏障影响的研究进展[J].中华中医药学刊,2010,28(8):1726.
• [2]王喜军.中药及中药复方的血浆药物化学研究[J].世界科学技术——中药现代化,2002,4(2):1.
• [3]王喜军.中药血浆药物化学的研究动态及发展趋势[J].中国中药杂志,2006,36(10):279.
• [4]周明眉,杨奎,王一涛.从血液流变学和血流速度评价大川芎丸方中川芎天麻治疗偏头痛的协同作用[J].中药药理与临床,2008,3(24):6.
• [5]袁莹,林晓,冯怡,等.川芎治疗偏头痛效应组分的体内移行研究[J].中国药学杂志,2010,45(9):694.
• [6]林晓,袁莹,洪燕龙,等.大川芎方中天麻效应组分的体内移行研究[J].中国中药杂志,2011,36(9):1175.