中国中药杂志

2012, v.37(13) 2017-2021

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大川芎方效应组分血浆及脑脊液HPLC特征指纹图谱研究
Study on HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid

沈岚;林晓;洪燕龙;梁爽;袁莹;冯怡;徐德生;阮克锋;
SHEN Lan1,2,LIN Xiao1,2,HONG Yanlong1,LIANG Shuang1,YUAN Ying1,FENG Yi1,XU Desheng3,RUAN Kefeng1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.College of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai

摘要(Abstract):

目的:通过大川芎方效应组分血浆及脑脊液HPLC指纹图谱的研究,建立准确、简便的体内定性分析方法,为大川芎方入血、入脑成分的研究奠定基础,对阐明其"引药上行"配伍规律提供依据。方法:建立大川芎方效应组分血浆及脑脊液HPLC指纹图谱。结果:各共有峰的相对保留时间的RSD<2.0%,6批血浆、脑脊液样品的指纹图谱的相似度均在0.85以上。结论:所建立的大川芎方效应组分血浆、脑脊液指纹图谱相对稳定、准确,可用于体内成分的定性分析。
Objective: To establish an accurate and simple qualitative analytical method in vivo in the study on HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid,in order to lay a foundation for studies on components found in plasma and cerebrospinal fluid and provide basis for compatible regularity of initiators.Method: HPLC characteristic fingerprint of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid was established.Result: The similarity of fingerprints of all of six batches of samples was above 0.85,RSD of their relative retention times in common peaks was less than 2.0%.Conclusion: The fingerprints of active components of Dachuanxiong Fang in plasma and cerebrospinal fluid were stable and accurate that they can be used in qualitative analysis on in vivo components.

关键词(KeyWords): 大川芎方效应组分;指纹图谱;血浆;脑脊液
active component of Dachuanxiong Fang;fingerprint;plasma;cerebrospinal fluid

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(30801548);; 国家“重大新药创制”科技重大专项(2009ZX09502-009);; 国家教育部科学技术研究重点项目(211060);; 上海市教育委员会科研创新项目(12ZZ124,11ZZ111);上海市教委重点学科项目(J50302);; 上海市自然基金项目(11ZR1434500)

作者(Author): 沈岚;林晓;洪燕龙;梁爽;袁莹;冯怡;徐德生;阮克锋;
SHEN Lan1,2,LIN Xiao1,2,HONG Yanlong1,LIANG Shuang1,YUAN Ying1,FENG Yi1,XU Desheng3,RUAN Kefeng1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.College of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 3.Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine,Shanghai

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