中国中药杂志

2008, (09) 996-998

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HPLC指纹图谱在金银花药材真伪鉴别中的应用研究
Application and study of HPLC fingerprint of Lonicera japonica for identification of geo-authenticity

向增旭;高山林;
XIANG Zeng-xu1,GAO Shan-lin2 (1.Iinstitute of Traditional Chinese Materials,Nanjing Agriculture University,Nanjing 210095,China;2.Traditional Chinese Medicinal College,China Pharmaceutical University,Nanjing 210038,China)

摘要(Abstract):

目的:研究金银花药材的指纹图谱测定方法,用指纹图谱的方法区别金银花道地和非道地药材。方法:色谱柱Hypersil ODS2(4.6 mm×150 mm,5μm),柱温20℃,流动相甲醇-0.05%磷酸溶液梯度洗脱,流速1 mL.min-1,检测波长238 nm。结果:道地药材共有18个共有峰,非道地产区药材共有9个共有峰。结论:HPLC指纹图谱能很好的区分道地和非道地金银花药材,为更好的控制金银花药材内在质量提供了科学依据。
Objective: To establish a chromatographic fingerprint of Lonicera japonica and apply it to identify geo-authentic of L.japonica.Method: The HPLC was applied in chromatographic separation and data were analysed by "computed aided similarity evaluation" software.Result: There exited distinct difference of chemical components group between and non-authentic samples.Conclusion: The established HPLC fingerprint can be used for the identification of geo-authentic of L.japonica.

关键词(KeyWords): 金银花;道地性;HPLC;指纹图谱
geo-authenticity;Lonicera japonica;fingerprint

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作者(Author): 向增旭;高山林;
XIANG Zeng-xu1,GAO Shan-lin2 (1.Iinstitute of Traditional Chinese Materials,Nanjing Agriculture University,Nanjing 210095,China;2.Traditional Chinese Medicinal College,China Pharmaceutical University,Nanjing 210038,China)

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