中国中药杂志

2020, v.45(21) 5226-5231

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千根草的化学成分研究
Study on chemical constituents from Euphorbia thymifolia

刘金龙;于敏;王素娟;张贵杰;
LIU Jin-long;YU Min;WANG Su-juan;ZHANG Gui-jie;College of Pharmacy, Guilin Medical University;

摘要(Abstract):

研究千根草Euphorbia thymifolia的化学成分。采用硅胶,Sephadex LH-20,MCI,ODS和制备型HPLC等色谱技术,从千根草地上部分的95%乙醇提取物中分离得到13个化合物,包括2个百里香酚类衍生物(1~2),4个生物碱(3~6),5个异香豆素(7~11)和2个鞣花酸(12~13),结构分别鉴定为:(Z)-8,9-dehydro-9,10-diisobutyryloxythymol(1),8-hydroxy-9,10-diisobutyryloxythymol(2),N-(N-benzoyl-L-phenylalanyl)-L-phenylalanol(3),aurantiamide acetate(4),1-carboethoxy-β-carboline(5),isoechinulin A(6),短叶苏木酚酸乙酯(7),euphorhirtin B(8),4,5-去氢诃子裂酸三乙酯(9),euphorhirtin G(10),pomegranatate(11),3,3′,4′-tri-O-methylellagic acid(12),3,3′-di-O-methylellagic acid(13)。化合物1为新化合物,化合物2,3和5~13均为首次从该植物中分离得到。对所有化合物进行了体外抗神经炎症活性筛选,结果显示化合物1~3和7对LPS激活的BV-2小胶质细胞释放NO具有显著抑制作用,IC_(50)分别为0.19,12.93,7.29,25.4μmol·L~(-1)。
This work was launched to study on the chemical constituents from Euphorbia thymifolia. Thirteen compounds were isolated from the 95% ethanol extract of the aerial parts of E. thymifolia by column chromatographies on silica gel, Sephadex LH-20, MCI, and ODS, and preparative HPLC, including two thymol derivatives(1-2), four alkaloids(3-6), five isocoumarins(7-11), together with two ellagic acids(12-13). All the compounds are listed as follows:(Z)-8,9-dehydro-9,10-diisobutyryloxythymol(1), 8-hydro-xy-9,10-diisobutyryloxythymol(2), N-(N-benzoyl-L-phenylalanyl)-L-phenylalanol(3), aurantiamide acetate(4), 1-carboethoxy-β-carboline(5), isoechinulin A(6), ethyl brevifolincarboxylate(7), euphorhirtin B(8), 4,5-didehydro chebulic acid triethyl ester(9), euphorhirtin G(10), pomegranatate(11), 3,3′,4′-tri-O-methylellagic acid(12), 3,3′-di-O-methylellagic acid(13). Compound 1 is a new compound. Except for compound 4, the others were isolated from this plant for the first time. All the compounds were screened for anti-neuroinflammatory activity in vitro, and compounds 1-3 and 7 showed significant activity with IC_(50) values of 0.19,12.93,7.29,25.4 μmol·L~(-1).

关键词(KeyWords): 千根草;化学成分;抗神经炎症活性
Euphorbia thymifolia;chemical constituents;anti-neuroinflammatory activity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21967007,21562017);; 广西自然科学基金项目(2018GXNSFAA138051)

作者(Author): 刘金龙;于敏;王素娟;张贵杰;
LIU Jin-long;YU Min;WANG Su-juan;ZHANG Gui-jie;College of Pharmacy, Guilin Medical University;

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DOI: 10.19540/j.cnki.cjcmm.20200703.201

参考文献(References):

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