中国中药杂志

2018, v.43(05) 879-884

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含挥发油饮片——姜黄饮片标准汤剂研究
Research on standard decoction of processed Curcumae Longae Rhizoma containing volatile oil

邓哲;张凡;焦梦姣;章军;石佳;程锦堂;刘安;
DENG Zhe;ZHANG Fan;JIAO Meng-jiao;ZHANG Jun;SHI Jia;CHENG Jin-tang;LIU An;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;Jiangxi University of Traditional Chinese Medicine;Shanghai University of Traditional Chinese Medicine;

摘要(Abstract):

制备姜黄饮片标准汤剂,建立其质量标准,为姜黄配方颗粒给予质量参照,同时也为含挥发油饮片的标准汤剂研究提供参考。依照含挥发油类中药饮片标准汤剂制备要求,制备了13批姜黄饮片标准汤剂,以姜黄素为定量指标成分,计算其转移率,测定干膏得率及pH并建立了HPLC图谱分析方法。13批姜黄饮片标准汤剂中姜黄素的转移率为0.67%~2.24%,干膏得率9.95%~18.36%,pH 3.86~6.05,并用中药色谱指纹图谱相似度评价系统(2012A)软件进行指纹图谱分析,确定了16个共有峰,指认3个,分别为双去甲氧基姜黄素(7号峰)、去甲氧基姜黄素(8号峰)和姜黄素(9号峰)。对13批姜黄饮片分别进行相似度评价,其相似度均大于0.78。同时,建立了姜黄饮片标准汤剂指纹图谱,该方法精密度、稳定性和重复性良好,具有一定的鉴别意义,适用于姜黄饮片配方颗粒质量控制。
To prepare processed Curcumae Longae Rhizoma standard decoction,establish its quality standard,provides quality reference for formulated Jianghuang granules and lay a foundation for standard decoction research for the pieces containing volatile oil.Thirteen batches of processed Curcumae Longae Rhizoma standard decoctions were prepared according to the preparation requirements for standard decoction of Chinese herbal medicine containing volatile oil. With curcumin as the detection index,the transfer rate and extraction ratio were calculated; the pH value was determined and HPLC fingerprint analysis method was established. The results showed that the transfer rate ranged from 0. 67% to 2. 24%; the extraction rate was at a range of 9. 95% to 18. 36% and pH value was3. 86 to 6. 05. Then the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 A) was used to analyze and compare the fingerprint. Sixteen common peaks were determined and three were identified including bisdemethoxycurcumin(peak 7),demethoxycurcumin(peak 8) and curcumin(peak 9). Moreover,the similarity was over 0. 78 in the similarity evaluation of 13 batches of samples. HPLC fingerprint analysis method of processed Curcumae Longae Rhizoma standard decoction was established,showing good precision,stability and repeatability with certain identification effect,suitable for the quality control of formulated Jianghuang granules.

关键词(KeyWords): 姜黄饮片;挥发油;标准汤剂;指纹图谱
processed Curcumae Longae Rhizoma;volatile oil;standard decoction;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家中药标准项目(ZYBZH-Y-JY-27);; 中国中医科学院“十三五”重点领域研究专项(ZZ10-007)

作者(Author): 邓哲;张凡;焦梦姣;章军;石佳;程锦堂;刘安;
DENG Zhe;ZHANG Fan;JIAO Meng-jiao;ZHANG Jun;SHI Jia;CHENG Jin-tang;LIU An;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;Jiangxi University of Traditional Chinese Medicine;Shanghai University of Traditional Chinese Medicine;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180105.013

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