中国中药杂志

2009, v.34(05) 620-624

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熟地等4味中药的植物雌激素作用的实验研究
Study on phytoestrogenic-like effects of four kinds of Chinese medicine including Radix Rehmanniae Preparata,Radix Paeoniae Alba,Radix Angelicae Sinensis,Rhizoma Chuanxiong

郝庆秀;王继峰;牛建昭;赵丕文;崔烨;孙丽萍;薛晓鸥;
HAO Qingxiu,WANG Jifeng*,NIU Jianzhao,ZHAO Piwen,CUI Ye,SUN Liping,XUE Xiaoou(Beijing University of Traditional Chinese Medicine,Beijing 100029,China)

摘要(Abstract):

目的:观察熟地黄、白芍、当归、川芎等中药对性未成熟SD大鼠子宫系数的影响,以及含药血清对人乳腺癌MCF7细胞体外增殖的影响,评价其植物雌激素样作用。方法:正常雌性性未成熟SD大鼠,体重(70±5)g,按体重均衡和随机的原则分为7组(正常对照、己烯雌酚、熟地黄、白芍、当归、川芎和四物汤组),分别每天分早、晚2次灌胃,给药均持续4 d,第5天腹主动脉取血,分离血清,分离子宫并称重,免疫组化法测定子宫组织雌激素受体表达量;药物血清用于MCF7细胞体外增殖,细胞周期和雌激素受体ERα蛋白表达的测定。结果:熟地黄、白芍和当归组的幼鼠子宫系数均高于正常对照组(P<0.05);川芎组幼鼠子宫系数与正常对照组相比,差异无统计学意义;白芍、当归、川芎的含药血清均可显著促进MCF7细胞MTT体外增殖(P<0.05),并且4味中药的药物血清均能使MCF7细胞S期细胞数和细胞增殖指数PI增加(P<0.01),当归和川芎可提高MCF7细胞ERα蛋白的表达水平(P<0.05),熟地黄和白芍的药物血清对MCF7细胞的ERα蛋白表达水平,与正常对照组相比,差异无统计学意义。结论:熟地黄、白芍和当归等中药的免煎剂有植物雌激素样作用,川芎组整体实验和体外细胞培养结果不完全一致,已烯雌酚在本实验剂量时有使幼鼠体重增长减缓的作用,而实验剂量的中药未表现出此样作用。
Objective: To explore the phytoestrogenic-like effects of four kinds of Chinese medicine including Radix rehmanniae preparata,Radix Paeoniae Alba,Radix Angelicae Sinensis,Rhizoma Chuanxiong.Method: Sixty immature female SD rats weighting(70±5) g were randomly divided into six groups: normal control group,positive control group and 4 Chinese medicine groups.The rats in different groups were treated for 4 days.On the fifth day,animals were sacrificed and uteri were separated solely and weighed.The blood was collected,and serum was separated.The effect of the pharmacological serum on proliferation assay with human breast cancer cell line(MCF7) by MTT method.Cell-cycle analyses were carried out with propidium iodide staining by flow cytometer.The expressions of subtypes-estrogen receptor-alpha(ERα) were detected by flow cytometry.Result: Radix Rehmanniae Preparata,Radix Paeoniae Alba and Radix Angelicae Sinensis could increase the immature rat's uterus wet weight and the ratio of uterus to body weight(P<0.05).The pharmacological serum of the four kinds of Chinese medicine stimulated proliferation of MCF7 cell respectively compared with normal control group(P<0.01).The cell cycle was impulsed from G1 to S,DNA synthesizing was inhanced,and PI was also increased.The pharmacological serum of Radix Angelicae Sinensis and Rhizoma Chuanxiong could increase the expressions of(ERα)(P<0.05).Conclusion: Radix Rehmanniae Preparata,Radix Paeoniae Alba and Radix Angelicae sinensis have phytoestrogenic effects.But the data weren't consistent with in vitro and in vivo assay of Rhizoma Chuanxiong.

关键词(KeyWords): 熟地黄;植物雌激素;子宫系数;MCF7;MTT;细胞周期;ERα
Radix Rehmanniae Preparata;phytoestrogen;rats;uterus coefficient;MCF7 cell;cell proliferation;cell-cycle analyses;ERα

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30510403202);; 教育部长江学者和创新团队发展计划项目(IRT0413);; 教育部博士点科研基金项目(20060026019);; 国家科技部支撑计划项目(2006BAI11B07)

作者(Author): 郝庆秀;王继峰;牛建昭;赵丕文;崔烨;孙丽萍;薛晓鸥;
HAO Qingxiu,WANG Jifeng*,NIU Jianzhao,ZHAO Piwen,CUI Ye,SUN Liping,XUE Xiaoou(Beijing University of Traditional Chinese Medicine,Beijing 100029,China)

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