中国中药杂志

2007, (12) 1171-1174

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金荞麦饮片液相色谱含量测定及指纹图谱研究
Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis

杨滨;辛敏通;吕冬梅;屈巧玲;李化;黄璐琦;
YANG Bin1,XIN Min-tong1,LU Dong-mei1,QU Qiao-ling1,2,LI Hua1,HUANG Lu-qi1(1.Institute of Chinese Materia Medica,China Academy of Traditional Chinese Medicine,Beijing 100700,China;2.Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

摘要(Abstract):

目的:研究金荞麦饮片高效液相色谱指纹图谱及含量测定方法。方法:采用高效液相色谱-电化学检测法,Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-0.1 mol.L-1磷酸盐缓冲液(pH 2.5);流速1.0 mL.min-1;柱温35℃;参比电极为ISAAC(in-situ Ag/AgC l);工作电极为玻碳电极;辅助电极为铂电极。结果:建立了金荞麦饮片高效液相色谱图谱,发现了6个色谱峰为共有峰,其中4个分别为没食子酸,原儿茶酸,原儿茶醛和表儿茶素的色谱峰。原儿茶酸的质量分数为0.004%~0.05%,原儿茶醛的质量分数为0.003%~0.015%。结论:该方法可用于金荞麦饮片质量控制。
Objective:To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.Method: The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis.The sample was performed on a column of Diamonsil C18(4.6 mm×250 mm,5 μm) which was eluted with methanol-0.1 mol·L-1 phosphate buffer(pH 2.5),the flow rate was 1.0 mL·min-1,the column temperature was 35 ℃,the reference electrode was ISAAC(in-situ silver/silver chloride),the work electrode was glassy carbon,the counter electrode was Pt platmun.Result: The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks,and gallic acid,protocatechuic acid,protocatechuic aldehyde and(-)-epicatechin were tested the samples.The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%,respectively.Conclusion: The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

关键词(KeyWords): 金荞麦饮片;高效液相色谱-电化学检测;含量测定;指纹图谱
Rhizoma Fagopyri Dibotoryis;HPLC-electrochemical detection;determination;fingerprint

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基金项目(Foundation): 北京市科技新星计划资助项目(H020821410190);; 中国博士后科学基金资助(2004035072)

作者(Author): 杨滨;辛敏通;吕冬梅;屈巧玲;李化;黄璐琦;
YANG Bin1,XIN Min-tong1,LU Dong-mei1,QU Qiao-ling1,2,LI Hua1,HUANG Lu-qi1(1.Institute of Chinese Materia Medica,China Academy of Traditional Chinese Medicine,Beijing 100700,China;2.Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

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