中国中药杂志

2019, v.44(24) 5441-5445

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

HPLC-ICP-MS同时检测6种砷形态的方法研究
Study on simultaneous determination of six arsenic species by HPLC-ICP-MS

李耀磊;王莹;王赵;金红宇;马双成;
LI Yao-lei;WANG Ying;WANG Zhao;JIN Hong-yu;MA Shuang-cheng;National Institutes for Food and Drug Control;

摘要(Abstract):

该研究旨在采用HPLC-ICP-MS建立同时测定砷甜菜碱(As B)、砷胆碱(As C)、二甲基砷(DMA)、亚砷酸根[As(Ⅲ)]、一甲基砷(MMA)和砷酸根[As(Ⅴ)]6种形态砷的检测方法。采用Dioncx Ion PacTMAS7阴离子交换柱(4 mm×250mm),以100 mmol·L-1碳酸铵(A)-1. 5 mmol·L-1磷酸氢二铵(B)为流动相,进行梯度洗脱,流速1 m L·min-1,进样量10μL。方法学结果显示,As B,As C,DMA,As(Ⅲ),MMA和As(Ⅴ)质量浓度在10~500μg·L-1线性关系良好(R2>0. 999);平均加样回收率(n=6)分别为105. 7%,100. 5%,102. 9%,105. 7%,100. 2%,92. 69%,RSD分别为0. 50%,2. 4%,0. 93%,1. 3%,0. 89%,1. 5%;此方法精密度与重复性均良好。在所建立的色谱条件下,6种形态砷均得到良好的分离;经方法学验证和实际样品测定,该方法可用于砷形态价态化合物的含量测定。
This study aims to establish a method for the determination of As B,As C,DMA,As( Ⅲ),MMA and As( Ⅴ) by using HPLC-ICP-MS. A Dioncx Ion PacTMAS7( 4 mm×250 mm) column was used for the HPLC-ICP-MS method. The mobile phase was 100 mmol·L-1 ammonium carbonate-1. 5 mmol·L-1 ammonium dibasic phosphate( gradient elution) at a flow rate of 1 m L·min-1. The injection volume was 10 μL. The linear relationships of As B,As C,DMA,As( Ⅲ),MMA,As( Ⅴ) were good with the concentration of10-500 μg·L-1. The average recovery rates( n = 6) were 105. 7%,100. 5%,102. 9%,105. 7%,100. 2%,92. 69%. The RSD were0. 50%,2. 4%,0. 93%,1. 3%,0. 89%,1. 5%. The precision and repeatability of this method were good. In this study,six forms of arsenic were separated effectively by this method. With methodological validation and sample determination,this method can be used to determine the morphological valence of arsenic in content determination.

关键词(KeyWords): 砷;形态价态;HPLC-ICP-MS;联用技术;亚砷酸根;砷酸根
arsenic;morphology of arsenic;HPLC-ICP-MS;hyphenated technique;As(Ⅲ);As(Ⅴ)

Abstract:

Keywords:

基金项目(Foundation): 国家“重大新药创制”科技重大专项(2014ZX09304307-002)

作者(Author): 李耀磊;王莹;王赵;金红宇;马双成;
LI Yao-lei;WANG Ying;WANG Zhao;JIN Hong-yu;MA Shuang-cheng;National Institutes for Food and Drug Control;

Email:

DOI:

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享