中国中药杂志

2013, v.38(02) 141-148

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中药材DNA条形码分子鉴定指导原则
Principles for molecular identification of traditional Chinese materia medica using DNA barcoding

陈士林;姚辉;韩建萍;辛天怡;庞晓慧;石林春;罗焜;宋经元;侯典云;石上梅;钱忠直;
CHEN Shi-lin1,2*,YAO Hui1,HAN Jian-ping1,XIN Tian-yi1,PANG Xiao-hui1, SHI Lin-chun1,LUO Kun1,SONG Jing-yuan1,HOU Dian-yun1,SHI Shang-mei3,QIAN Zhong-zhi3(1.The National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China; 2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 3.Chinese Pharmacopoeia Commi

摘要(Abstract):

随着中药材DNA条形码分子鉴定方法研究的深入与普及,国家药典委员会讨论通过在《中国药典》增补本中列入中药材DNA条形码分子鉴定指导原则,该指导原则通过对大样本量中药材进行DNA条形码分子鉴定研究,建立以ITS2为核心,psbA-trnH为辅的植物类药材DNA条形码鉴定体系和以COI为主、ITS2为辅的动物类药材DNA条形码鉴定体系。该文介绍了中药材DNA条形码分子鉴定指导原则及其起草说明,并对该原则在中药材鉴定方面的应用前景进行展望。
Since the research of molecular identification of Chinese Materia Medica(CMM) using DNA barcode is rapidly developing and popularizing,the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China.Based on the study on comprehensive samples,the DNA barcoding systems have been established to identify CMM,i.e.ITS2 as a core barcode and psbA-trnH as a complementary locus for identification of planta medica,and COI as a core barcode and ITS2 as a complementary locus for identification of animal medica.This article introduced the principle of molecular identification of CMM using DNA barcoding and its drafting instructions.Furthermore,its application perspective was discussed.

关键词(KeyWords): 中国药典;中药材;DNA条形码;分子鉴定;指导原则
Chinese pharmacopoeia;traditional Chinese materia medica;DNA barcoding;molecular identification;principle

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基金项目(Foundation): 国家自然科学基金项目(81130069);; 国家高技术研究发展计划(863)项目(2012AA021602);; 教育部长江学者和创新团队发展计划项目(IRT1150)

作者(Author): 陈士林;姚辉;韩建萍;辛天怡;庞晓慧;石林春;罗焜;宋经元;侯典云;石上梅;钱忠直;
CHEN Shi-lin1,2*,YAO Hui1,HAN Jian-ping1,XIN Tian-yi1,PANG Xiao-hui1, SHI Lin-chun1,LUO Kun1,SONG Jing-yuan1,HOU Dian-yun1,SHI Shang-mei3,QIAN Zhong-zhi3(1.The National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China; 2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 3.Chinese Pharmacopoeia Commi

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