中国中药杂志

2014, v.39(12) 2300-2304

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HPLC同时测定不同产地茄根中2种苯丙酰胺类成分的含量
HPLC determination of two phenylpropanoid amides in Solani Melongenae Radix from different cultivation regions

孙晶;顾宇凡;李曼曼;苏小琴;霍会霞;张静;黄正;张倩;赵云芳;李军;屠鹏飞;
SUN Jing;GU Yu-fan;LI Man-man;SU Xiao-qin;HUO Hui-xia;ZHANG Jing;HUANG Zheng;ZHANG Qian;ZHAO Yun-fang;LI Jun;TU Peng-fei;Modern Research Center for Traditional Chinese Medicine,Beijing University of Chinese Medicine;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

摘要(Abstract):

建立同时测定茄根中2种苯丙酰胺类成分N-反式-对香豆酰基去甲辛弗林和N-反式-对香豆酰基酪胺含量的高效液相色谱方法。采用Agilent Eclipse XDB C18色谱柱(4.6 mm×250 mm,5μm);以甲醇-0.1%甲酸水溶液为流动相梯度洗脱,流速1.0 mL·min-1;柱温30℃;检测波长300 nm。结果表明,N-反式-对香豆酰基去甲辛弗林和N-反式-对香豆酰基酪胺色谱峰分离良好,分别在2.84~68.16,3.10~74.40 mg·L-1呈良好的线性关系,平均加样回收率(n=9)分别为99.30%,102.8%。该方法快速、准确、重复性好,可用于茄根药材的质量控制。
A quantitative HPLC-DAD method was developed for simultaneous determination of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine in Solani Melongenae Radix from different cultivation regions in China. The separation was performed on an Agilent Eclipse XDB C18column(4. 6 mm × 250 mm,5 μm) at 30 ℃ with a gradient elution of methanol and 0. 1% formic acid in water as mobile phase. The flow rate was set at 1. 0 mL·min-1and the detection wavelength was 300 nm. The calibration curves of N-trans-p-coumaroyloctopamine and N-trans-p-coumaroyltyramine were linear over the ranges of 2. 84-68. 16,3. 10-74. 40 mg·L-1,and the average recoveries(n =9) were 99.30% and 102.8%,respectively. The developed method was successfully applied for the analysis of sixteen samples from different cultivation regions in China,which indicated that the method is simple,rapid,accurate,and reliable for quality evaluation of Solani Melongenae Radix.

关键词(KeyWords): 茄根;N-反式-对香豆酰基去甲辛弗林;N-反式-对香豆酰基酪胺;苯丙酰胺衍生物;高效液相色谱;质量控制
Solani Melongenae Radix;N-trans-p-coumaroyloctopamine;N-trans-p-coumaroyltyramine;phenylpropanoid amide;HPLC;quality control

Abstract:

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基金项目(Foundation): 国家工信部中药材扶持基金项目;; 2015年版《中国药典》项目

作者(Author): 孙晶;顾宇凡;李曼曼;苏小琴;霍会霞;张静;黄正;张倩;赵云芳;李军;屠鹏飞;
SUN Jing;GU Yu-fan;LI Man-man;SU Xiao-qin;HUO Hui-xia;ZHANG Jing;HUANG Zheng;ZHANG Qian;ZHAO Yun-fang;LI Jun;TU Peng-fei;Modern Research Center for Traditional Chinese Medicine,Beijing University of Chinese Medicine;School of Chinese Materia Medica,Beijing University of Chinese Medicine;

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