中国中药杂志

2003, (06)

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6种决明属药用植物种子毛细管电泳法鉴别
Identification of Six Species of Cassia Seeds by Capillary Electrophoresis

宋贤丽,郭宝林,刘克武,潘映红
SONG Xian-li 1,3 , GUO Bao-lin 2, LIU Ke-wu 1, PAN Ying-hong 3 (1.College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100094, China; 3. State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, China)

摘要(Abstract):

目的 :利用毛细管电泳鉴别六种豆科决明属药用植物种子。方法 :采用高效毛细管电泳对六种决明属植物种子中的水溶性成分进行分析 ,电泳分离缓冲液为 0 .1mol·L-1SDS ,0 .1mol·L-1硼酸盐 (pH =8.5 ) ,运行电压为 2 5kV。结果 :确定 4个峰为 6种决明属植物种子的共有峰 ,并得到各自的特征峰。结论 :6种决明属植物种子的毛细管电泳指纹图谱有明显的差异 ,可利用这些差异对其进行鉴别。
Objective: To identify Cassia seeds of six species by capillary electrophoresis. Method: The water-soluble extracts of Cassia seeds of six species were analyzed by capillary electrophoresis. The running buffer was 0.1 mol·L -1 borate, 0.1 mol·L -1 SDS, pH 8.5. The separation voltage was 25 kV. Result: Four common peaks could be found in the electropherograms of six species Cassia seeds, and the characteristic peaks could also be observed. Conclusion: Fingerprints of the six species of Cassia seeds show significant differences, which can be used for their identification.

关键词(KeyWords): 决明属;种子;毛细管电泳;指纹图谱;鉴别
Cassia ; seeds; capillary electrophoresis; fingerprint; identification

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基金项目(Foundation): 科技部重大项目前期研究专项(2 0 0 1CCA0 110 0 )资助

作者(Author): 宋贤丽,郭宝林,刘克武,潘映红
SONG Xian-li 1,3 , GUO Bao-lin 2, LIU Ke-wu 1, PAN Ying-hong 3 (1.College of Life Science, Sichuan University, Chengdu 610064, Sichuan, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100094, China; 3. State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100094, China)

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