中国中药杂志

2011, v.36(09) 1175-1178

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大川芎方中天麻效应组分的体内移行研究
In vivo transmigration of active ingredients from Gastrodia elata in Dachuanxiong Fang

林晓;袁莹;洪燕龙;徐德生;沈岚;冯怡;
LIN Xiao1,2,YUAN Ying2,HONG Yanlong1,XU Desheng3,SHEN Lan2,FENG Yi1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese medicine of Ministryof Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.School of Pharmacy,Shanghai University of Traditional Chinese medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai 200021,China)

摘要(Abstract):

目的:借鉴血清药物化学的方法,探讨大川芎方中天麻的药效物质基础,为有效、全面地控制大川芎方及其制剂的质量奠定基础。方法:通过HPLC指纹图谱,比较空白血浆与含药血浆,空白脑脊液与含药脑脊液的成分异同,确定天麻效应组分的体内移行成分,并应用HPLC-DAD-MSn分析技术推测移行成分的化学结构。结果:灌胃大川芎方效应组分后,血浆中较明显来源于天麻效应组分的色谱峰有2个,经推断分别为天麻素和巴利森苷类成分;而脑脊液中未明显检测到来源于天麻效应组分的色谱峰。结论:天麻体内移行成分研究有助于阐明其效应物质基础。
Objective:To investigate the substance basis of Gastrodia elata in Dachuanxiong Fang(DF) by serum pharmacochemistry,so that the quality of DF as well as its preparations can be effectively and systemically controlled.Method: The chromatographic peaks of blank plasma,blank cerebrospinal fluid,drug-contained plasma and cerebrospinal fluid were compared by HPLC fingerprints.The absorbed ingredients of the active parts extracted from G.elata were structurally identified by HPLC-DAD-MSn.Result: Two components,which were definitely detectable in rat plasma following the oral administration of the active parts from G.elata,were identified as gastrodin and parishin.In rat cerebrospinal fluid,there are,however,no obvious peaks that belong to the ingredients from G.elata.Conclusion: Research on in vivo transmigration of ingredients from G.elata is favorable to the clarification of its active substance basis.

关键词(KeyWords): 天麻;效应组分;移行成分;物质基础
Gastrodia elata;active components;migrated constituent;substance basis

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2009ZX09502-009);; 上海市科委项目(09DZ2292000);; 上海市教育委员会科研创新项目(09YZ117,11zz112);上海市教育委员会重点学科资助项目(J50302);; 上海中医药大学杏林学者培养计划项目(R110212)

作者(Author): 林晓;袁莹;洪燕龙;徐德生;沈岚;冯怡;
LIN Xiao1,2,YUAN Ying2,HONG Yanlong1,XU Desheng3,SHEN Lan2,FENG Yi1(1.Engineering Research Center of Modern Preparation Technology of Traditional Chinese medicine of Ministryof Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.School of Pharmacy,Shanghai University of Traditional Chinese medicine,Shanghai 201203,China;3.Shuguang Hospital,Shanghai 200021,China)

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