中国中药杂志

2007, (20) 2146-2149

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乌骨藤药材脂溶性成分的GC指纹图谱研究
Studies on characteristic fingerprints of fat-soluble components of Marsdenia tenacissima by GC

赵陆华;相秉仁;李晓晶;杨丽莉;
ZHAO Lu-hua1,2,XIANG Bing-ren1,2,LI Xiao-jing1,2,YANG Li-li3 (1.Key Laboratory of Drug Quality Control and Pharmacovilance Ministry of Education,Nanjing 210009,China;2.Center for Instrumental Analysis,China Pharmaceutical University,Nanjing 210009,China;3.Manjing Environmental Monitoring Center,Nanjing 210013,China)

摘要(Abstract):

目的:采用GC和GC-MS色谱法研究并建立各产地乌骨藤药材脂溶性成分的指纹图谱。方法:采用毛细管气相色谱分析,DB-5石英毛细管柱(0.25 mm×30 m,0.25μm);程序升温,柱温70℃(保持5 min),以8℃.min-1升至200℃(保持12 min),再以5℃.min-1升至260℃(保持10 min);以高纯氮为载气;氢火焰离子化检测器(FID),检测器温度300℃;气化室温度280℃;分流比10∶1。结果:乌骨藤药材指纹图谱选定了15个共有峰,质谱分析鉴定出其成分,并对不同产地药材进行聚类分析及相似度评价。结论:本法重现性好,可结合乌骨藤药材的HPLC指纹图谱评价其质量。
Objective:To study characteristic fingerprints of the fat-soluble components of different Marsdenia tenacissima samples by GC and GC-MS.Method: The sample was split in the 280 ℃ injection port,with 10∶1 split ratio,and separated on a fused silica capillary column(DB-5,30 m×0.25 mm I.D.,0.25 μm film).The temperature program was 70 ℃ for 5 min,8 ℃·min-1 to 200 ℃,then 5 ℃·min-1 to 260 ℃,260 ℃ for 10 min,The high pure nitrogen was used as a carrier gas,The FID temperature was 300 ℃.Result:There are 15 common peaks in the GC fingerprints of M.tenacissima and they are identified by GC-MS.The analyses on cluster and similarity degree of the finger the crude drugs from various habitats have been performed.Conclusion: The method has good repeatability.The GC fingerprint combined with the HPLC fingerprint of M.tenacissim can be used to evaluate its quality integrally.

关键词(KeyWords): 乌骨藤;脂溶性成分;指纹图谱;GC;GC-MS
Marsdenia tenacissima;fat-soluble components;fingerprint;GC;GC-MS

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基金项目(Foundation): 江苏省自然科学基金项目(BK2005100)

作者(Author): 赵陆华;相秉仁;李晓晶;杨丽莉;
ZHAO Lu-hua1,2,XIANG Bing-ren1,2,LI Xiao-jing1,2,YANG Li-li3 (1.Key Laboratory of Drug Quality Control and Pharmacovilance Ministry of Education,Nanjing 210009,China;2.Center for Instrumental Analysis,China Pharmaceutical University,Nanjing 210009,China;3.Manjing Environmental Monitoring Center,Nanjing 210013,China)

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