朱丹;赵鑫;徐俏;宁伟;
ZHU Dan,ZHAO Xin,XU Qiao,NING Wei(College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture,Ministry ofEducation,College of Animal Science,Shenyang Agricultural University,Shenyang 110866,China)

• 1:东北野菜种质异位保存圃与鉴定中心
• 2:沈阳农业大学园艺学院设施园艺省部共建教育部重点实验室

摘要(Abstract)：

目的:运用RP-HPLC建立中药蒲公英指纹图谱,为提高蒲公英的质量控制手段提供依据。方法:采用Kromasil100-5 C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(含0.5%冰乙酸)系统梯度洗脱,检测波长为323 nm,流速为1.0 mL.min-1,柱温35℃,进样量10μL,测定东北地区11种蒲公英指纹图谱。结果:用梯度洗脱得到的色谱图中,多数峰都可以达到较好分离。11种蒲公英指纹图谱可检测出25个相对位置稳定的共有峰,通过与标准品的保留时间及紫外光谱比较,10号峰(1),12号峰(2),16号峰(3),25号峰(4)分别鉴定为绿原酸、咖啡酸、对香豆酸和木犀草素。该分析方法具有很好的精密度、重复性和稳定性,符合指纹图谱相关要求。结论:方法准确可靠,可用于蒲公英药材的质量的控制。
Objective: To study the RP-HPLC fingerprints of 11 plants in the genus Taraxacum for their quality control.Method: The fingerprints were determined using an Agilent 1100 series instrument system.Chromatographic analyses were performed on a Kromasil 100-5 C18(4.6 mm×250 mm,5 μm) analytical column,eluted with methanol and water containing 0.5% acetic acid as the mobile phases in gradient elution at the flow rate of 1.0 mL·min-1.The detection wavelength was 323 nm.The temperature of column was 35 ℃.Eleven species of Taraxacum in northeast of China were detected respectively.Result: Twenty-five common peaks existed in 11 RP-HPLC fingerprints.By comparing the retention time and the on-line UV spectra,peaks No.10,No.12,No.16 and No.25 were identified as chlorogenic acid,caffeic acid,p-coumaroy acid and luteolin respectively.Conclusion: The analytical method with good precision and reproducibility can be useful in the quality control of Taraxacum plants.

关键词(KeyWords)： 蒲公英;指纹图谱
Taraxacum;HPLC fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家农业部项目[农计函(2010)58号]

作者(Author): 朱丹;赵鑫;徐俏;宁伟;
ZHU Dan,ZHAO Xin,XU Qiao,NING Wei(College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture,Ministry ofEducation,College of Animal Science,Shenyang Agricultural University,Shenyang 110866,China)

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DOI:

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