中国中药杂志

2012, v.37(04) 426-433

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黄芩不同规格与化学成分及内在质量相关性的研究
Research on relationship between commercial specifications of Scutellariae Radix and chemical composition and drug quality

王丹;蒋亚杰;梁艳;赵琳;蔡少青;尚明英;袁鹏飞;赵玉英;王璇;
WANG Dan 1,JIANG Yajie 1,2,LIANG Yan1,ZHAO Lin 1,CAI Shaoqing 2,SHANG Mingying 2, YUAN Pengfei 2,ZHAO Yuying 2,WANG Xuan 1(1.Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China; 2.State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China)

摘要(Abstract):

目的:比较4种不同规格黄芩化学成分差异,研究枯心部位对药材化学成分的影响,为该药材生产、加工、销售、临床各环节的质量评价提供科学依据。方法:采用中药材鉴定学方法对不同规格黄芩药材的外观性状进行观察,采用HPLC指纹图谱方法对不同规格黄芩药材及枯心、非枯心部位的化学成分进行检测。结果:不同规格黄芩药材直径、长度以及枯心程度差异明显,因芩王、条芩一级样品全部可见枯心,作者将其分类为枯芩,条芩二级、末等品内部坚实,可分类为子芩。发现黄芩同产地不同规格样品指纹谱轮廓相似,但特征色谱峰相对峰面积有差异,枯芩的3对黄酮苷元和苷成分(黄芩素与黄芩苷、汉黄芩素与汉黄芩苷、千层纸素A与千层纸素A苷)的峰面积比值显著高于子芩。枯心部分的HPLC指纹谱总峰面积显著低于非枯心部分,但一些特征峰含量和3对成分的峰面积比值却高于非枯心部分,可视为区分枯芩和子芩的特征参数。结论:不同规格黄芩可分类为枯芩和子芩。枯芩和子芩化学成分特征不同,有必要进一步探讨二者药效的异同。该研究结果可为不同规格黄芩鉴别和质量控制及枯芩、子芩药效学物质基础研究提供参考依据。
Objective:To compare the chemical differences in 4 commercial specifications of Scutellaria Radix,research the affection of decayed central xylem part on the crude drug's chemical composition and provide scientific data for production,processing,sale and clinical applications of Scutellariae Radix.Method:Macroscopical identification method was used for observation of different specifications of Scutellariae Radix,including Qinwang,Tiaoqin both in 1st class and 2nd class and inferior samples.HPLC fingerprint method was used to analyze chemically the decayed central xylem part and non-decayed part as well as complete sample,and the results were described by the relative peak area.Result:The morphological characteristics of 4 specifications are greatly different from one another mainly in root diameters,root lengths and the proportions of decayed central xylem part in the root,and so the authors classified Qinwang and Tiaoqin in 1st class as Kuqin for all samples of them which have decayed central xylem;and classified Tiaoqin in 2nd class and the inferior samples as Ziqin,for having little decayed central xylem.The 4 specifications collected from the same producing area have similar HPLC fingerprint profile to one another,while they are different in relative peak area.The peak area ratios of aglycone to their glucuronide(baicalein/baicalin,wogonin/wogonoside,oroxylin A/oroxylin A-7-O-glucuronide) from Kuqin were significantly higher than those of Ziqin.The total area of the peaks in HPLC fingerprint chromatographs of decayed central xylem part were quite lower than that of non-decayed part,whereas peak areas of the characteristic peaks and the 3 peak area ratios of decayed central xylem were significantly higher than those of non-decayed part which could be used as characteristic parameters to distinguish Kuqin and Ziqin.Conclusion:Four commercial specifications of Scutellariae Radix can be classified as Kuqin and Ziqin respectively according to morphological characteristics and the proportions of decayed central xylem part in the root.The chemical characteristics of Kuqin and Ziqin are different from each other,so it's worth clarifying the similarities and differences of Kuqin and Ziqin in future.The result in this research can be used as references for identification and quality control of Scutellariae Radix specifications,and investigation on effective components of Kuqin and Ziqin.

关键词(KeyWords): 黄芩规格;性状;HPLC指纹图谱;黄酮;枯芩;子芩
commercial specifications of Scutellariae Radix;morphological characteristics;HPLC fingerprint;flavonoids;Kuqin;Ziqin

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973)项目(2006CB504700);; 中医药行业科研专项项目(200807019)

作者(Author): 王丹;蒋亚杰;梁艳;赵琳;蔡少青;尚明英;袁鹏飞;赵玉英;王璇;
WANG Dan 1,JIANG Yajie 1,2,LIANG Yan1,ZHAO Lin 1,CAI Shaoqing 2,SHANG Mingying 2, YUAN Pengfei 2,ZHAO Yuying 2,WANG Xuan 1(1.Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China; 2.State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China)

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