丁小余,徐珞珊,王峥涛,徐红,周开亚
DING Xiao yu 1, 2 , XU Luo san 1 , WANG Zheng tao 1, XU Hong 1, ZHOU Kai ya 2 (1.Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiangsu China; 2.College of Life Sciences, Nanjing Normal University, Nanj

• 1:中国药科大学中药学院
• 2:中国药科大学中药学院
• 3:中国药科大学中药学院
• 4:中国药科大学中药学院

摘要(Abstract)：

目的 :探讨束花石斛与同属相似种 :玫瑰石斛、喇叭唇石斛、报春石斛、兜唇石斛等植物及药材鉴别的DNA分子标记。方法 :对束花石斛及其相似种进行rDNAITS区序列比较研究。结果 :尽管束花石斛及其相似种的外部形态差异较小 ,但在rDNAITS序列上却存在着显著而稳定的差异。在rDNAITS区域中 ,作者共挑选了 15个碱基位点用作鉴别束花石斛及其相似种的DNA标记。结论 :根据rDNAITS区碱基序列差异 ,能准确鉴别束花石斛及其相似种植物及药材
Objective: To define molecular characters to distinguish D. chrysanthum from its allied species D. primulinum, D. lituiflorum,D. aphyllum,D. crepidatum. Method: The molecular characteristics of D. chrysanthum and its allied species were compared. The sequences of rDNA ITS regions were exploited to explore the evidence for authentication D. chrysanthum and its allied species. Result: Although the morphological difference was slight, the sequence difference of ITS regions among five rDNAs was obvious and stable. Fifteen sites of ITS region were defined as DNA character to identify D. chrysanthum from the other four allied species. Conclusion: The difference of rDNA ITS sequences can be used to authenticate accurately D. chrysanthum from three allied species of Dendrobium .

关键词(KeyWords)： 束花石斛;喇叭唇石斛;报春石斛;兜唇石斛;玫瑰石斛;rDNAITS区序列;DNA序列鉴别
D. chrysanthum; D. lituiflorum;D. primulinum; D. aphyllum;D. crepidatum ; sequences of rDNA ITS region; authentication by DNA sequence

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目 (3 0 17114 4)

作者(Author): 丁小余,徐珞珊,王峥涛,徐红,周开亚
DING Xiao yu 1, 2 , XU Luo san 1 , WANG Zheng tao 1, XU Hong 1, ZHOU Kai ya 2 (1.Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiangsu China; 2.College of Life Sciences, Nanjing Normal University, Nanj

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