中国中药杂志

2008, (01) 51-53

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青葙子药材的HPLC指纹图谱分析
HPLC fingerprints in seed of Celosia argentea

王莹;郭美丽;王笑康;殷军;
WANG Ying1,2,GUO Mei-li1,WANG Xiao-kang2, YIN Jun2(1.Department of Pharmacognosy,College of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.Department of Pharmacognosy,College of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

摘要(Abstract):

目的:研究不同产地青葙子药材的HPLC指纹图谱,为青葙子药材的鉴别及质量控制提供依据。方法:采用Elite Hypersil ODS2(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.1%冰醋酸水溶液为流动相梯度洗脱,流速1mL.min-1,以蒸发光散射检测器(ELSD)检测,记录70 min色谱图。结果:22个不同产地青葙子药材的指纹图谱相似度较高,有12个共有峰,各共有峰之间的分离度较好;聚类分析将产于我国中部地区的青葙子聚为1类,而产于我国南部地区的青葙子被聚为另1类。结论:青葙子药材指纹图谱的特征性及专属性强,可用于青葙子药材的鉴别及质量控制;不同产地青葙子的化学组成存在一定差异,固定产地来源对保障青葙子药材质量的稳定性极为重要。
For preferable authentication and regulation of material quality of Celosia argentea,HPLC fingerprints of different habitats were studied.Analysis was carried out on a Hypersil ODS2 column(4.6 mm×250 mm,5 μm) with acetonitrile-0.1% glacial acetic acid as the mobile phase,and eluates were detected by an evaporative light scattering detector(ELSD).The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(Version 2004 A) was applied to analyses the similarity of the fingerprint of diverse habitats.The similarity results were verified by SPSS. The chromatographic profiles of the samples from different regions were very similar.HPLC fingerprints of Semen C.argentea 12 common peaks and each peak in the fingerprint was well separated under the chromatographic condition above.The different habitats of C.argentea can be grouped to two types: the middle region and the south region.The chemical constituents of C.argentea vary with different habitats so selection of material habitat is very important for quality control of C.argentea.The fingerprint with high individuality and specificity could be applied in the identification and quality control of the material of C.argentea.

关键词(KeyWords): 青葙子;HPLC-ELSD;指纹图谱
Celosia argentea;HPLC-ELSD;fingerprint

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基金项目(Foundation): 上海市中药现代化专项(06DZ19715);; 军队“十一五”中医药研发推广专项(2006172003)

作者(Author): 王莹;郭美丽;王笑康;殷军;
WANG Ying1,2,GUO Mei-li1,WANG Xiao-kang2, YIN Jun2(1.Department of Pharmacognosy,College of Pharmacy,Second Military Medical University,Shanghai 200433,China;2.Department of Pharmacognosy,College of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

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