中国中药杂志

2004, (02)

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参乌胶囊对β-淀粉样蛋白大鼠模型外周血淋巴细胞DNA的影响
Effect of Shenwu Capsule on the peripheral blood lymphocyte DNA of rat model induced by β-amyloid injection

王辉,李林,叶翠飞,张兰
WANG Hui,LI Lin,YE Cui-fei,ZHANG Lan ( Department of Pharmacology,Xuanwu Hospital of Capital University of Medical Sciences,Beijing Key Laboratory for Brain Aging,Beijing,100053,China )

摘要(Abstract):

目的 :观察中药新复方参乌胶囊对 β 淀粉样蛋白海马内注射大鼠模型外周血淋巴细胞DNA损伤和血清脂质过氧化物丙二醛 (MDA)水平的影响。方法 :大鼠单侧 (左侧 )海马内注射Aβ2 5-3 57d后 ,利用单细胞电泳结合激光共聚焦显微技术对外周血淋巴细胞DNA图象进行分析 ,通过硫代巴比妥酸法检测血清MDA含量。结果 :参乌胶囊灌胃给药 2周能够明显降低彗星样淋巴细胞的比率、尾长和尾距 ,降低血清MDA水平 ,保护淋巴细胞DNA免受氧化损伤的影响。结论 :参乌胶囊对外周血淋巴细胞DNA具有保护作用。
Objective: To investigate the effect of the new Traditional Chinese Compound Shenwu Capsule on the damage of lymphocyte DNA and lipid peroxidation in peripheral blood of rats induced by β-amyloid injection. Method: The animal model was made by injection of β-amyloid 25-35 into hippocampus of rats. DNA damage of lymphocytes was measured by the single cell gel electrophoresis (SCGE) combined with the laser scanning confocal microscopy (LSCM). The content of malondialdehyde (MDA) was determined by TBA assay. Result: Shenwu Capsule decreased the rate of the comet-like cell,comet-like cell lengh (TCL),and tail moment (TM) of lymphocytes in peripheral blood of model rats. Shenwu Capsule also declined the MDA content in serum of Aβ model rats. Conclusion: Shenwu Capsule has protective effect on peripheral blood lymphocyte DNA.

关键词(KeyWords): 中药复方;Alzheimer病;β-淀粉样蛋白;淋巴细胞DNA;脂质过氧化
Traditional Chinese Compound; Alzheimer's disease; β-amyloid; lymphocyte DNA; lipid peroxidation

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划“ 973”项目(G2 0 0 0 0 5 70 10 ) ;; 北京市重点科技实验室项目 (95 180 60 0 ) ;; 北京市科技项目 (95 2 60 180 0 ) ;; 北京市中医药重点学科项目 (2 0 0 1) ;; 北京市自然科学基金项目 (70 3 2 0 13 )

作者(Author): 王辉,李林,叶翠飞,张兰
WANG Hui,LI Lin,YE Cui-fei,ZHANG Lan ( Department of Pharmacology,Xuanwu Hospital of Capital University of Medical Sciences,Beijing Key Laboratory for Brain Aging,Beijing,100053,China )

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