中国中药杂志

2006, (04) 301-303

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不同产地枳壳麸炒品HPLC指纹图谱研究
Study on HPLC fingerprint of different growing areas on Fructus Aurantii fried with bran

曹君;龚千锋;肖兵华;王少军;王跃生;杨世林;
CAO Jun~(1),GONG Qian-feng~(1),XIAO Bing-hua~(1),WANG Shao-jun~(2),WANG Yue-sheng~(2),YANG Shi-lin~(2)(1.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;2.National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Nanchang 330077,China)

摘要(Abstract):

目的:建立枳壳麸炒品HPLC指纹图谱分析方法。方法:用HPLC测定,以醋酸乙酯超声法提取样品,梯度洗脱,紫外检测波长320 nm,柱温30℃,流速1 mL.min-1。以柚皮苷为参照物分析了10批不同产地的枳壳麸炒品,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004 A进行评价,建立了共有模式,并以10批枳壳麸炒品相关系数评价指纹的相似性。结果:枳壳麸炒品液相色谱指纹图由11个特征峰构成指纹图谱的特征。所建立的指纹图谱具有稳定、重复的特点。结论:该方法准确、简单,适用于枳壳麸炒品分析,色谱指纹图谱可用于枳壳麸炒品的鉴别和质量控制。
Objective: To establish a method for HPLC fingerprint determination of different growing areas on Fructus Aurantii fried with bran.Method: HPLC chromatographic experiment was carried out by ultrasonic distilment of acetacetic ester,gradient elution mode and the detection wavelength of 320 nm.The column temperature was set at 30 ℃,and flow rate is 1.0 mL·min~(-1).Naringin served as the reference standard.Ten batches of Fructus Aurantii fried with bran in the different places were analyzed.Similarity evaluation system for chromatographic fingerprint of TCM proceeded the evaluation,The correlation coefficients between ten batches were used to define the similarity.Result: There were eleven characteristic peaks in Fructus Aurantii fried with bran by HPLC fingerprint which constituted fingerprint characters.Both the reproducibility and stability on HPLC fingerprint were proper.Conclusion: The method was accurate and simple.It can be applied to the analysis on Fructus Aurantii fried with bran.The fingerprint spectrum can be used to distinguish Fructus Aurantii fried with bran and to be its quality control.

关键词(KeyWords): 枳壳麸炒品;柚皮苷;HPLC指纹图谱
Fructus Aurantii fried with bran;naringin;HPLC fingerprint

Abstract:

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基金项目(Foundation): 国家“十五”科技攻关项目(2001BA701A55-26)

作者(Author): 曹君;龚千锋;肖兵华;王少军;王跃生;杨世林;
CAO Jun~(1),GONG Qian-feng~(1),XIAO Bing-hua~(1),WANG Shao-jun~(2),WANG Yue-sheng~(2),YANG Shi-lin~(2)(1.Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;2.National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine,Nanchang 330077,China)

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