中国中药杂志

2011, v.36(06) 775-779

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藤黄药材的指纹图谱研究
Fingerprint analysis of gamboge by HPLC

侯文洁;陈保来;萧伟;
HOU Wenjie1,3,CHEN Baolai2,3,XIAO Wei2,3*(1.Nanjing University of Chinese Medicine,Nanjing 210046,China; 2.Jangsu Kanion pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine,Lianyungang 222001,China; 4.Enterprises Academician Workstations,Lianyungang 222001,China)

摘要(Abstract):

目的:建立藤黄药材的高效液相指纹图谱方法。方法:采用Luna C8(4.6 mm×250 mm,5μm)色谱柱,柱温25℃,以乙腈-0.1%冰乙酸水溶液为流动相梯度洗脱,检测波长362 nm,流速为1.0 mL·min-1。采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对11批不同批次的藤黄药材指纹图谱进行相似度计算。结果:各批藤黄药材中有13个共有峰,各峰分离度良好,各批次藤黄药材间共有峰的相对保留时间RSD均<1.0%,药材间相似度均>90%。结论:本方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于藤黄药材的真伪鉴别和质量综合评价。
Objective: To establish the chromatographic fingerprint for the quality control of gamboge.Method: Analysis on a Luna C8(4.6 mm×250 mm,5 μm)column eluted with mobile phases containing acetonitrile and 0.1% glacial acetic acid in water in gradient mode.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 362 nm.The temperature of column was 25 ℃.And data of 11 batches of gamboge samples from different sources were analysed by "similarity evaluation for chromatographic Fingerprint of Traditional Chinese Medicine" software.Result: Thirteen common peaks were selected in chromatograms,and all the common peeks were separated effectively.Conclusion: The precision,repeatability,and stability of this method were satisfying.The method developed can be used to identify and evaluate the quality of gamboge.

关键词(KeyWords): 藤黄;HPLC;指纹图谱
gamboge;HPLC;fingerprint

Abstract:

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基金项目(Foundation): 国家“重大新药创制”科技重大专项(2008ZX09101-Z-024)

作者(Author): 侯文洁;陈保来;萧伟;
HOU Wenjie1,3,CHEN Baolai2,3,XIAO Wei2,3*(1.Nanjing University of Chinese Medicine,Nanjing 210046,China; 2.Jangsu Kanion pharmaceutical Co.,Ltd.,Lianyungang 222001,China; 3.State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine,Lianyungang 222001,China; 4.Enterprises Academician Workstations,Lianyungang 222001,China)

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