中国中药杂志

2019, v.44(15) 3316-3322

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基于UPLC指纹图谱及化学计量学的不同产地白芍差异比较研究
Comparative study on differences of Paeonia lactiflora from different habitats based on fingerprint and chemometrics

赵秋龙;卞晓坤;钱大玮;张婷;朱振华;郭盛;严辉;王团结;陈志鹏;段金廒;
ZHAO Qiu-long;BIAN Xiao-kun;QIAN Da-wei;ZHANG Ting;ZHU Zhen-hua;GUO Sheng;YAN Hui;WANG Tuan-jie;CHEN Zhi-peng;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine;National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of C

摘要(Abstract):

通过指纹图谱研究比较不同产地白芍药材之间的差异。建立白芍药材的UPLC指纹图谱,对收集的四川、河北、河南、山西和安徽5个白芍主要产地样品进行分析,并利用UPLC-Q-TOF/MS对峰进行指认。采用《中药色谱指纹图谱相似度评价系统》软件结合偏最小二乘判别分析(PLS-DA)对共有峰相对峰面积进行分析。色谱指纹图谱有12个共有峰,通过对照品指认、文献比对初步鉴定了其中10个成分,所有样品与对照图谱的相似度均大于0. 900,但经PLS-DA分析,白芍样品按产地被明显地聚集为5组,并通过变量权重重要性排序(VIP)值图发现了峰2、峰6(没食子酸乙酯)、峰10(galloypaeoniflorin)、峰12(苯甲酰芍药苷)为5个产地白芍样品之间的主要差异成分。研究发现不同产地白芍药材虽然所含成分种类基本一致,但从整体上分析评价,其中的4个成分在含量上存在一定的差异,研究结果可为白芍的用药选择和不同产地白芍的综合利用提供参考。
This study aims to compare the differences of Paeonia lactiflora from different habitats by establishing fingerprint. The fingerprint of P. lactiflora was established by UPLC. The samples collected from Sichuan,Hebei,Henan,Shanxi and Anhui were analyzed. The common peaks were identified by UPLC-Q-TOF/MS. The relative peak area of the common peaks was analyzed through similarity evaluation system( 2012 edition) for chromatographic fingerprint of traditional Chinese medicine developed by the National Pharmacopoeia Commission. Twelve common peaks were obtained and ten components were identified by reference substance and literature comparison. The similarity of each sample to the reference fingerprint is greater than 0. 900. However,all samples were clearly divided into 5 groups according to habitats after PLS-DA analysis. The peaks 2,6( ethyl gallate),10( galloypaeoniflorin) and 12( benzoyl paeoniflorin) were found to be the main difference components between the samples from five different habitats through the VIP value map. The study found that the variety of ingredients in the different areas are basically similar. But there are some differences in the content of the four components. The results of this study can provide reference at choosing and utilizing P. lactiflora from different places comprehensively.

关键词(KeyWords): 白芍;指纹图谱;化学计量学;偏最小二乘法判别分析;差异成分
Paeonia lactiflora;fingerprint;chemometrics;PLS-DA;difference component

Abstract:

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基金项目(Foundation): 国家中药标准化项目(ZYBZH-C-JS-28);; 宁夏回族自治区重点研发计划(东西部科技合作)项目(2017BY079)

作者(Author): 赵秋龙;卞晓坤;钱大玮;张婷;朱振华;郭盛;严辉;王团结;陈志鹏;段金廒;
ZHAO Qiu-long;BIAN Xiao-kun;QIAN Da-wei;ZHANG Ting;ZHU Zhen-hua;GUO Sheng;YAN Hui;WANG Tuan-jie;CHEN Zhi-peng;DUAN Jin-ao;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine;National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of C

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