中国中药杂志

2020, v.45(14) 3467-3474

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基于HS-GC-MS及化学计量学的不同形态龙血树属含脂药材的成分差异比较研究
Comparative study on differences of resin-containing drugs in Dracaena from different appearance based on HS-GC-MS and chemometrics

苏晶;李宜航;周玲娟;秦天道;刘世芳;陈曦;李光;马巾媛;
SU Jing;LI Yi-hang;ZHOU Ling-juan;QIN Tian-dao;LIU Shi-fang;CHEN Xi;LI Guang;MA Jin-yuan;Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture;Xishuangbanna Dai Autonomous Prefecture People′s Hospital;College of Tropical Crops, Yunnan Agricultural University;

摘要(Abstract):

以4种不同形态的龙血树属含脂药材为研究对象,采用顶空进样-气相色谱-质谱联用(HS-GC-MS)的代谢组学技术和层次聚类分析(HCA)的化学计量学的方法,研究不同形态龙血树属含脂药材中挥发性成分差异,并进行代谢通路分析。偏最小二乘法判别分析(PLS-DA)和HCA分析结果表明,富含纤维的样品和中空木栓层样品中挥发性成分差异不大,但与通体含树脂样品和分泌树脂聚集的样品中的挥发性成分存在较大的代谢差异,并以PLS-DA的VIP和P筛选出20种差异代谢物,在通体含树脂样品和分泌树脂聚集的样品中含量明显高于在富含纤维的样品和中空木栓层样品的含量。富集差异代谢物的代谢通路(P<0.05)共16条,主要涉及萜类生物生成和苯丙烷类代谢,该结果为深入研究不同形态的龙血树属含脂药材结脂机制提供了参考,同时为建立多指标质量评价体系提供了借鉴。
Resin-containing drugs in Dracaena from four different appearances were analyzed by headspace sampling-gas chromatography-mass spectrometry(HS-GC-MS) metabolomics technique and hierarchical clustering analysis(HCA) chemometrics method. This study was to analyze differential volatile components in resin-containing drugs in Dracaena from different appearance and metabolic pathways. The results of partial least squares discriminant analysis(PLS-DA) and HCA analysis indicated that there was little difference in volatile components between fiber-rich sample and hollow cork cambium sample, however, the volatile components in the two samples compared with whole body resin-containing sample and resin-secreting aggregated sample had a large metabolic difference. Twenty differential metabolites were screened by VIP and P values of PLS-DA. The content of these differential metabolites was significantly higher in whole body resin-containing sample and resin-secreting aggregated sample than in fiber-rich sample and hollow cork cambium sample. Sixteen significant metabolic pathways were obtained through enrichment analysis(P<0.05), mainly involved in terpenoids biosynthesis and phenylpropanoid metabolism. This result provided a reference for further study of resin formation mechanism of resin-containing drugs in Dracaena from different appearances. At the same time, it also provided a reference for establishing a multi-index quality evaluation system.

关键词(KeyWords): 龙血树属含脂药材;HS-GC-MS;化学计量学;龙血竭
resin-containing drugs in Dracaena;HS-GC-MS;chemometrics;dragon′s blood

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81503289);; 云南省基础研究面上项目(2016FB143);; 云南省技术创新人才培养项目(2017HB097);; 中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-012);; 云南省工信委中药饮片产业发展专项(2019-YZ-065)

作者(Author): 苏晶;李宜航;周玲娟;秦天道;刘世芳;陈曦;李光;马巾媛;
SU Jing;LI Yi-hang;ZHOU Ling-juan;QIN Tian-dao;LIU Shi-fang;CHEN Xi;LI Guang;MA Jin-yuan;Yunnan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College;Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture;Xishuangbanna Dai Autonomous Prefecture People′s Hospital;College of Tropical Crops, Yunnan Agricultural University;

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