中国中药杂志

2018, v.43(05) 977-984

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基于指纹图谱和多组分含量测定的艾叶药材质量控制研究
Quality evaluation of Artemisiae Argyi Folium based on fingerprint analysis and quantitative analysis of multicomponents

郭龙;焦倩;张丹;刘爱朋;王乾;郑玉光;
GUO Long;JIAO Qian;ZHANG Dan;LIU Ai-peng;WANG Qian;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease,Hebei University of Chinese Medicine;

摘要(Abstract):

艾叶菊科植物艾Artemisa argyi的干燥叶,具有温经止血,散寒止痛之功效,为临床常用中药。艾叶中含有挥发油、有机酸和黄酮等多种成分。相比于艾叶中的挥发油类成分,对其非挥发性成分的研究较少。该文针对艾叶中的非挥发性成分,采用高效液相色谱法对其进行了分析,建立了艾叶非挥发性成分化学指纹图谱,共标定了10个共有峰,艾叶样品的相似度均在0.940之上,所建立的艾叶指纹图谱专属性强,可用于艾叶药材的质量评价。在此基础上,对艾叶及其混伪品野艾叶药材中的新绿原酸、绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、棕矢车菊素、异泽兰黄素7种指标性成分进行了含量测定。基于含量测定结果,采用聚类分析和主成分分析等化学计量学方法对艾叶和野艾叶药材进行了区分与比较。结果表明艾叶与其混伪品野艾叶药材化学成分间存在差异,所建立的多组分定量分析方法可用于正品艾叶药材的鉴别与质量控制。
Artemisiae Argyi Folium,the dried leaves of Artemisia argyi,has been widely used in traditional Chinese and folk medicines for treatment of hemorrhage,pain,and skin itch. Phytochemical studies indicated that volatile oil,organic acid and flavonoids were the main bioactive components in Artemisiae Argyi Folium. Compared to the volatile compounds,the research of nonvolatile compounds in Artemisiae Argyi Folium are limited. In the present study,an accurate and reliable fingerprint approach was developed using HPLC for quality control of Artemisiae Argyi Folium. A total of 10 common peaks were marked,and the similarity of all the Artemisiae Argyi Folium samples was above 0. 940. The established fingerprint method could be used for quality control of Artemisiae Argyi Folium. Furthermore,an HPLC method was applied for simultaneous determination of seven bioactive compounds including five organic acids and two flavonoids in Artemisiae Argyi Folium and Artemisiae Lavandulaefoliae Folium samples. Moreover,chemometrics methods such as hierarchical clustering analysis and principal component analysis were performed to compare and discriminate the Artemisiae Argyi Folium and Artemisiae Lavandulaefoliae Folium based on the quantitative data of analytes. The results indicated that simultaneous quantification of multicomponents coupled with chemometrics analysis could be a well-acceptable strategy to identify and evaluate the quality of Artemisiae Argyi Folium.

关键词(KeyWords): 艾叶;指纹图谱;含量测定;化学计量学;质量控制
Artemisiae Argyi Folium;fingerprint analysis;quantitative analysis;chemometrics methods;quality evaluation

Abstract:

Keywords:

基金项目(Foundation): 河北省现代农业产业技术体系中药材产业创新团队建设项目(2017041458-2);; 孙宝惠全国名老中医专家传承工作室项目(7002016008005);; 中央本级重大增减支项目(2060302);; 河北省中医药管理局科研计划项目(2018/08)

作者(Author): 郭龙;焦倩;张丹;刘爱朋;王乾;郑玉光;
GUO Long;JIAO Qian;ZHANG Dan;LIU Ai-peng;WANG Qian;ZHENG Yu-guang;College of Pharmacy,Hebei University of Chinese Medicine;Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease,Hebei University of Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.2018.0029

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