许舜军;李鹏;杨柳;张勉;王峥涛;
XU Shun-jun~(1),LI Peng~(2),YANG Liu~(3),ZHANG Mian~(1),WANG Zheng-tao~(1)(1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China;2.Guangzhou Post and Telegraph Hospital,Guangzhou 510630,China;3.Second Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510120,China)

• 1:中国药科大学生药学研究室
• 2:广州市邮电医院
• 3:广东省中医院中心实验室
• 4:中国药科大学生药学研究室

摘要(Abstract)：

目的:建立牡丹皮的高效液相色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法:利用HPLC-DAD,梯度洗脱,测定了30批牡丹皮药材样品,并应用LC-MS和MS-MS技术指认指纹图谱中的主要色谱峰。结果:30批牡丹皮样品得到的色谱指纹图谱有15个共有峰,指认了色谱峰的化学归属,主要特征峰的峰面积排序依次为:丹皮酚>五没食子酰葡萄糖>没食子酸甲酯>没食子酰芍药苷>没食子酸>羟基芍药苷>其他色谱峰。结论:牡丹皮的指纹图谱特征性及专属性强,可结合含量测定用于全面控制牡丹皮的质量。
Objective: To establish a HPLC fingerprint for quality evaluation of Cortex Moutan.Method: The HPLC chromatographic fingerprinting of 30 lots of Cortex Moutan was established and major peaks were identified by LC-MS and MS-MS.Result: The HPLC fingerprint of Cortex Moutan was established,showing 15 characteristic peaks.The areas of these peaks were found to complied with the following rule: paeonol > 1,2,3,4,6-penta-O-galloyl-glucos > methyl gallate > galloylpaeoniflorin > gallic acid > oxypaeoniflorin > other compounds.Conclusion: The chromatographic fingerprinting of Cortex Moutan with high specificity can be used to control its quality and monitor lot to lot consistency.

关键词(KeyWords)： 牡丹皮;高效液相色谱;指纹图谱
Cortex Moutan;HPLC;fingerprinting

Abstract:

Keywords:

基金项目(Foundation): 国家中医药管理局重点资助项目(国中医药科2001ZDZX005)

作者(Author): 许舜军;李鹏;杨柳;张勉;王峥涛;
XU Shun-jun~(1),LI Peng~(2),YANG Liu~(3),ZHANG Mian~(1),WANG Zheng-tao~(1)(1.Department of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China;2.Guangzhou Post and Telegraph Hospital,Guangzhou 510630,China;3.Second Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510120,China)

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DOI:

参考文献(References)：

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