中国中药杂志

2019, v.44(15) 3221-3225

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铁皮石斛非淀粉多糖含量测定方法的研究
Study on quantification method of non-starch polysaccharides in Dendrobium catenatum

李娅;刘京晶;张新凤;吴世华;李绍平;斯金平;
LI Ya;LIU Jing-jing;ZHANG Xin-feng;WU Shi-hua;LI Shao-ping;SI Jin-ping;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University;Zhejiang University;University of Macau;

摘要(Abstract):

为了精准、简便测定铁皮石斛葡甘聚糖为主的非淀粉多糖类主要活性成分,更好的指导优质药材的生产、加工与评价,建立了"水提—α-淀粉酶处理—一次醇沉"处理方法,与2015年版《中国药典》法比较,测得总多糖、葡萄糖、甘露糖含量分别下降了20. 9%,58. 8%,1. 6%,能够有效的去除淀粉,而葡甘聚糖等非淀粉类活性多糖仅损耗1. 3%;经方法学考察验证,重复性、稳定性良好,适用于铁皮石斛药材非淀粉多糖含量测定,可作为现行药典法含量测定的替代方法,对其他含淀粉药材的非淀粉多糖的测定也有借鉴作用。
The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum. In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation. The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method. The contents of starches,non-starch polysaccharides and monosaccharides were analyzed. In addition,the system suitability was tested. Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20. 9%,58. 8% and 1. 6% respectively. The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable. Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum. Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.

关键词(KeyWords): 铁皮石斛;α-淀粉酶处理;非淀粉多糖;单糖组成
Dendrobium catenatum;α-amylase treatment;non-starch polysaccharides;monosaccharide composition

Abstract:

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基金项目(Foundation): 国家重点研发计划项目(2017YFC1702201);; 国家自然科学基金项目(81603228)

作者(Author): 李娅;刘京晶;张新凤;吴世华;李绍平;斯金平;
LI Ya;LIU Jing-jing;ZHANG Xin-feng;WU Shi-hua;LI Shao-ping;SI Jin-ping;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University;Zhejiang University;University of Macau;

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