中国中药杂志

2010, v.35(06) 732-735

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黄芪药材RRLC-UV-MS指纹图谱研究
RRLC-UV-MS fingerprint of Radix Astragali

苏娴;吴立军;屠鹏飞;
SU Xian1,2,WU Lijun1,TU Pengfei2*(1.Department of Traditional Chinese Medicine & Pharmacognosy,Shenyang PharmaceuticalUniversity,Shenyang 110016,China;2.Department of Natural Medicines,School of Pharmaceutical Sciences,Peking University HealthScience Center,Beijing 100083,China)

摘要(Abstract):

目的:建立黄芪的RRLC/UV-MS指纹图谱分析方法。方法:采用Agilent Zorbax Extend C18色谱柱(2.1 mm×100mm,1.8μm);以乙腈-0.1%甲酸为流动相进行梯度洗脱;流速0.3 mL.min-1,进样量0.5μL;柱温30℃;检测波长254 nm。结果:建立了黄芪药材RRLC/UV-MS指纹图谱分析方法,对11批样品进行了分析,其相似度均达到0.92以上,并对其中7个色谱峰进行了初步归属。结论:本方法快速高效,可用于黄芪指纹图谱的测定,并为其全面质量控制提供参考。
Objective: To establish a method for detecting the fingerprint of Radix Astragali by RRLC-UV-MS.Method: Separation was performed on an Agilent Zorbax Extend C18 column(2.1 mm×100 mm,1.8 μm).Gradientelution was performed by the mobile phases consisting of acetonitrile and 0.1% formic acid with the flow rate of 0.3 mL ·min-1,the detection wavelength was set at 254 nm,and the column temperature was 30 ℃.Result: The method of fingerprint analysis on Radix Astragali by RRLC-UV-MS was established.Eleven samples of Radix Astragali were analyzed,the similarities were over 0.92,and seven peaks in the fingerprint were designated.Conclusion: The method can be applied to the quality control and studies on chemical constituents of Radix Astragali.

关键词(KeyWords): 黄芪;指纹图谱;RRLC-MS
Radix Astragali;fingerprint;RRLC-UV-MS;chemical constituents

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基金项目(Foundation): 国家自然科学基金杰出青年科学基金项目(30525043)

作者(Author): 苏娴;吴立军;屠鹏飞;
SU Xian1,2,WU Lijun1,TU Pengfei2*(1.Department of Traditional Chinese Medicine & Pharmacognosy,Shenyang PharmaceuticalUniversity,Shenyang 110016,China;2.Department of Natural Medicines,School of Pharmaceutical Sciences,Peking University HealthScience Center,Beijing 100083,China)

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