中国中药杂志

2009, v.34(19) 2485-2488

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缙云黄芩HPLC指纹图谱的建立及其不同部位活性成分含量研究
Analysis of fingerprint and bioactive components in different parts of Scutellaria tsinyunensis by HPLC

张家辉;杨蕊;邓洪平;
ZHANG Jiahui,YANG Rui,DENG Hongping(Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE),School of Life Science,Southwest University,Chongqing 400715,China)

摘要(Abstract):

目的:建立重庆特有药用植物缙云黄芩HPLC指纹图谱,对其根、茎、叶中4种主要的黄酮类活性成分进行分离、含量测定和比较研究。方法:采用梯度洗脱方法进行色谱分离和含量测定,记录10个种群缙云黄芩指纹图谱,采用国家药典委"中药色谱指纹图谱相似度评价系统2004A版"建立指纹图谱共有模式。结果:建立了同时分离和测定4种活性成分的梯度洗脱程序;缙云黄芩指纹图谱14个共有峰分离度良好、保留时间稳定、重现性好;供试样品间相似度较高;4种活性成分总量多少依次为根>茎>叶,尤其是黄芩苷在根中的积累量达茎中的5倍以上。结论:本研究建立的色谱指纹图谱分析方法稳定、可靠,能为缙云黄芩种源的宏观分析、探明其遗传变异模式及其保护和合理开发利用等提供重要的理论依据。
Objective: Used HPLC to set up fingerprint of medicinal-plant Scutellaria tsinyunensis collected from Jinyun mountain of Chongqing,and to determine four flavonoids in its root,stem and leaf.Method: Analysis was carried out on a COSMOSIL C18-PAQ column(4.6 mm×250 mm,5 μm) with acetonitrile and H2O-H3PO4-THF(85∶12∶0.3) as the mobile phases in a gradient elution,with a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃,and the detection wavelength was 275 nm.The fingerprints of ten populations were determined,and the data were analyzed by the similarity evaluation software.Result: The mutual mode of HPLC fingerprint was established.There were 14 common peaks in all,each peak was well separated.The peak was steady and showed good repeatability.Compared with the mutual mode,the similarities of 10 samples were 0.987,0.994,0.993,0.971,0.983,0.994,0.981,0.981,0.988,0.972,and the average similarity was 0.984.The chromatographic profiles of 10 samples were very similar.The four flavonoids were determined successfully by this method.The results showed that the content of flavonoids in the root was the most,and the leaf was the least.Conclusion: This method was steady and reliable which could be applied in identification,genetic diversity analysis,protection and utilization of S.tsinyunensis.

关键词(KeyWords): 缙云黄芩;HPLC;指纹图谱;黄芩苷;黄芩素;汉黄芩苷;汉黄芩素
Scutellaria tsinyunensis;HPLC;fingerprint;baicalin;daicalein;wogonoside;wogonin

Abstract:

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基金项目(Foundation): 重庆市自然科学基金计划项目(CSTC,2008BB5256);; 国家“211工程”三期建设项目

作者(Author): 张家辉;杨蕊;邓洪平;
ZHANG Jiahui,YANG Rui,DENG Hongping(Key Laboratory of Eco-environments in Three Gorges Reservoir Region(MOE),School of Life Science,Southwest University,Chongqing 400715,China)

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