中国中药杂志

2010, v.35(13) 1731-1734

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

野生与栽培防风中升麻苷和5-O-甲基维斯阿米醇苷的含量测定及指纹图谱分析
Determination of prim-O-glucosylcimifugin and 5-O-methylvisamm isoide in Saposhnicovia divaricata and HPLC fingerprint analysis

马丽杰;杨滨;冯学锋;殷小杰;李化;格小光;朱立革;曹军;
MA Lijie1,2,YANG Bin1,FENG Xuefeng1,YIN Xiaojie1,LI Hua1,GE Xiaoguang1,ZHU Lige3,CAO Jun1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Department of Pharmarcology,Inner Mongolia Medical College,Hohhot 010059,China;3.Heilongjiang University of Chinese Medicine,Harbin 150040,China)

摘要(Abstract):

目的:建立并比较野生和栽培品种防风中升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法和HPLC指纹图谱。方法:含量测定采用岛津C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(40∶60),检测波长254 nm,流速1.0 mL.min-1,柱温30℃。HPLC指纹图谱测定采用资生堂MGⅡC18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,柱温30℃。结果:升麻苷加样回收率为99.6%,RSD 0.72%(n=6);5-O-甲基维斯阿米醇苷加样回收率为102.6%,RSD 0.88%。野生与栽培品种升麻苷质量分数分别为(4.96±2.59),(3.61±1.82)mg.g-1;5-O-甲基维斯阿米醇苷质量分数分别为(3.91±2.09),(4.37±2.02)mg.g-1。野生与栽培品种升麻苷和5-O-甲基维斯阿米醇苷质量分数无显著性差异。在此条件下所建立的指纹图谱中防风各成分得到有效分离。结论:升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法简便,结果准确,HPLC指纹图谱分析可作为防风药材质量控制及分类评价的依据。
Objective: To develop HPLC methods for the determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnicovia divaricata and of HPLC fingerprint to compare the wild and culture varieties.Method: Conditions of determination: Shimadzu C18 column(4.6 mm×150 mm,5 μm),methanol-water(40∶60) as mobile phase with the flow rate of 1 mL·min-1.The detection wavelength was 254 nm.Conditions of HPLC fingerprint: MG Ⅱ C18 column(4.6 mm ×250 mm,5 μm),the mobile phase was acetonitrile-water with the flow rate of 1 mL·min-1,using linear gradient elution,the column temperature was 30 ℃.Result: The average recovery of prim-O-glucosylcimifugin was 99.6%(RSD 0.72%,n=6).The average recovery of 5-O-methylvisammisoide was 102.6%(RSD 0.88%,n=6).The contents of prim-o-glucosylcimifugin in wild and culture varieties were(4.96±2.59) and(3.61±1.82) mg·g-1 respectively.The contents of 5-O-methylvisammisoide were(3.91±2.09) and(4.37±2.02) mg·g-1 respectively.The compositions of S.divaricata were effective separated under the conditions of HPLC fingerprint.Conclusion: The HPLC determination method of prim-O-glucosylcimifugin and 5-O-methylvisammisoide is convenient and accurate.The HPLC fingerprint analysis method could be a basis for quality control and classification evaluate of S.divaricata.

关键词(KeyWords): 防风;升麻苷;5-O-甲基维斯阿米醇苷;指纹图谱
Saposhnicovia divaricata;prim-O-glucosylcimifugin;5-O-methylvisammisoide;fingerprint

Abstract:

Keywords:

基金项目(Foundation): 科研院所社会公益研究专项(2005DIA1J172)

作者(Author): 马丽杰;杨滨;冯学锋;殷小杰;李化;格小光;朱立革;曹军;
MA Lijie1,2,YANG Bin1,FENG Xuefeng1,YIN Xiaojie1,LI Hua1,GE Xiaoguang1,ZHU Lige3,CAO Jun1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Department of Pharmarcology,Inner Mongolia Medical College,Hohhot 010059,China;3.Heilongjiang University of Chinese Medicine,Harbin 150040,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享