中国中药杂志

2004, (02)

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葛根素对大鼠胰岛素刺激下骨骼肌细胞膜GLUT4蛋白含量的影响
Effects of puerarin on plasma membrane GLUT4 content in skeletal muscle from insulin-resistant sprague-dawley rats under insulin stimulation

宋春宇,毕会民
SONG Chun-yu,BI Hui-min (Department of Endocrinology,Renmin Hospital of Wuhan University,Wuhan 430060, China)

摘要(Abstract):

目的 :了解葛根素对糖尿病胰岛素抵抗状态是否有改善作用 ,并探讨这种作用是否通过影响大鼠骨骼肌细胞膜GLUT4蛋白含量而实现。方法 :制备胰岛素抵抗大鼠模型。将动物分为 3组 :正常对照组 ;胰岛素抵抗模型组 ;胰岛素抵抗模型 +葛根素治疗组。第 3组大鼠腹腔注射葛根素 10 0mg·kg-1·d-1,治疗 4周。用Westernblot方法检测骨骼肌细胞膜表面GLUT4蛋白表达。结果 :胰岛素抵抗模型组大鼠骨骼肌细胞膜GLUT4蛋白表达显著减少 ,与正常对照组相比 ,减少约 31% ;用葛根素注射液治疗后 ,细胞膜GLUT4蛋白表达明显上调 ,约 1.18倍。结论 :葛根素可能通过降糖、改善高胰岛素血症 ,从而影响细胞内胰岛素信号传导 ,使GLUT4转位至细胞膜增加 ,其在膜上的含量也增加 ,发挥快速转运葡萄糖的作用。
Objective: To explore the effect of puerarin injection on the amount of GLUT4 protein at the plasma membrane in insulin-resistant rat skeletal muscle. Method: The rat model of insulin resistance (IR) was made by being fod with high-fat diet. The animals were divided into three groups (ten in each group):group I:controls;group II:Insulin-resistant rats;group III:Insulin-resistant rats+Puerarin treatment. Insulin-resistant rats were injected with 100 mg puerarin injection per kg body weight through abdominal cavity once a day for 4 weeks. Fasting blood glucose and fasting serum insulin levels were measured before and after Puerarin treatment,respectively. Insulin treatment was achieved by intraperitoneal injection of insulin(1 unit insulin per kg body weight.)15 minute before killing the animals. The right hindlimb skeletal muscle was rapidly dissected. Then the expression of GLUT4 protein at the plasma membrane in all the animals was assessed with Western blot method. Result: The GLUT4 content at the plasma membrane in insulin-resistant rats skeletal muscle was significantly lower(about 31%) than that of controls( P <0.01).Puerarin Injection partly corrected fasting blood glucose(from 6.17±0.67 mmol·L -1 to 5.54±0.35 mmol·L -1 ) and fasting serum insulin levels(from 17.09±2.02 mU·L -1 to 11.86±1.35 mU·L -1 )and increased the GLUT4 content at the plasma membrane by 1.18-fold in insulin-resistant rats skeletal muscle. Conclusion: Puerarin Injection can ameliorate IR,and the mechanism may be involved in increasing cell-surface level of GLUT4 through decreasing fasting blood glucose and fasting serum insulin levels,improving GLUT4 trafficking and intracellular insulin signaling.

关键词(KeyWords): 葛根素;胰岛素抵抗;骨骼肌;葡萄糖转运体4
puerarin; insulin resistance(IR); skeletal muscle; glucose transporter 4(GLUT4)

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作者(Author): 宋春宇,毕会民
SONG Chun-yu,BI Hui-min (Department of Endocrinology,Renmin Hospital of Wuhan University,Wuhan 430060, China)

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