炮制前后豨莶草指标性成分及指纹图谱研究Study on index components and fingerprints of crude and processed Siegesbeckia Herbs
孔繁瑶;胡慧华;田志浩;徐文英;冯孟鑫;马长华;
KONG Fan-yao;HU Hui-hua;TIAN Zhi-hao;XU Wen-ying;FENG Meng-xin;MA Chang-hua;Beijing University of Chinese Medicine;
摘要(Abstract)：
该文通过研究豨莶草生品及炮制一至九制品中奇壬醇、豨莶精醇和豨莶苷含量变化规律及指纹图谱对比,为豨莶草炮制研究提供实验基础。采用SunFire-C18色谱柱(4.6 mm×150 mm,5μm),柱温30℃,流动相乙腈(含0.1%甲酸)-水(含0.1%甲酸),流速1 mL·min-1,进样量30μL,检测波长215,320 nm,奇壬醇在2.180~26.16 mg·L-1呈良好的线性关系,平均加样回收率为96.4%,RSD 2.2%;豨莶精醇在2.900~34.80 mg·L-1呈良好的线性关系,平均加样回收率为97.2%,RSD 1.7%;豨莶苷在1.012~6.072 mg·L-1呈良好的线性关系,平均加样回收率为96.3%,RSD 2.4%。该方法准确可靠,重复性好,快速简便;实验结果为豨莶草炮制过程中化学成分变化规律和进一步阐明其疗效的改变奠定了基础。
The change of kirenol,darutigenol and darutoside in Siegesbeckia and its first to ninth processed products were studied,and the ten fingerprints were compared,which provided the experimental basis for the study of Siegesbeckia processing technology.The samples were analysed by HPLC on a SunFire-C18column(4.6 mm × 150 mm,5 μm) with gradient elution of acetonitrile(0.1% formic acid)-water(0.1% formic acid) at a flow rate of 1.0 mL·min- 1.Column temperature was 30 ℃ and the detected wavelength was 215,320 nm.The calibration curves of kirenol,darutigenol and darutoside were linear in the range of 2.180-26.16,2.900-34.80,and 1.012-6.072 mg · L- 1,respectively,and the average recoveries were 96.4%,97.2% and 96.3% wit RSD2.2%,1.7% and 2.4%.This method was simple,the result was stable and had good repeatability,recovery and precision.The result was the basis of the chemical contents variation in the processing of Siegesbeckia Herbs and further clarifying the effect of the changing.
关键词(KeyWords)：
豨莶草;奇壬醇;豨莶精醇;豨莶苷;中药炮制
Siegesbeckia Herbs;darutigenol;darutoside;kirenol;preparation
基金项目(Foundation): 国家自然科学基金项目(81173550)
作者(Author):
孔繁瑶;胡慧华;田志浩;徐文英;冯孟鑫;马长华;
KONG Fan-yao;HU Hui-hua;TIAN Zhi-hao;XU Wen-ying;FENG Meng-xin;MA Chang-hua;Beijing University of Chinese Medicine;
Email:
DOI:
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