中国中药杂志

2013, v.38(09) 1401-1405

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菊苣和毛菊苣的HPLC指纹图谱构建及模式识别研究
HPLC fingerprint and chemical pattern recognition method for Cichorium intybus and C. glandulosum

周俊;张冰;林志健;吴丽丽;朱文静;孙博喻;
ZHOU Jun1,ZHANG Bing1,LIN Zhi-jian1,WU Li-li2,ZHU Wen-jin1,SUN Bo-yu1(1.School of Pharmacology,Beijing University of Traditional Chinese Medicine,Beijing 100102,China; 2.Department of Pharmacy,the Hospital of Ganzhou,Ganzhou 341000,China)

摘要(Abstract):

目的:建立菊苣和毛菊苣地上部分的HPLC指纹图谱,为鉴别菊苣品种和有效控制菊苣质量提供参考。方法:采用高效液相色谱法采集了来自全国14个省市11批菊苣和6批毛菊苣样品的指纹图谱,按"中药色谱指纹图谱相似度评价系统"建立对照图谱,并对样品进行相似度评价。运用SPSS软件对不同品种菊苣的HPLC指纹图谱特征峰进行了聚类分析和主成分分析。结果:成功建立菊苣和毛菊苣指纹图谱,11批菊苣样品相似度均大于0.874,6批毛菊苣样品相似度均大于0.9。聚类分析和主成分分析均实现了菊苣和毛菊苣HPLC指纹图谱差异的识别。结论:该方法简便、可靠,可用于菊苣、毛菊苣的品种鉴别和菊苣的质量评价。
Objective: To establish fingerprint analysis method by HPLC for the quality control of Cichorium intybus and effective identification of C.intybus and C.glandulosum.Method: The software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs"(Version 2004A) was employed to generate the mean chromatogram and carry out the similarity analysis of the samples.Cluster analysis was adopted in combination with principal component analysis to study seventeen chicory′s characteristic common peaks and to differentiate the two chicory resources.Result: The fingerprint of C.intybus and C.glandulosum has been set up,and the range of similarity for seventeen chicory samples was 0.847-0.988.The difference among chromatographic fingerprints of chicory samples between the two different varieties was identified by cluster analysis and principal component analysis.Conclusion: The method can be used to evaluate the quality of C.intybus and identify C.intybus and C.glandulosum conveniently.

关键词(KeyWords): 菊苣;指纹图谱;HPLC;质量控制;品种鉴别
chicory;fingerprints;HPLC;quality control;variety identification

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(81073068,81274153);; 北京中医药大学科研创新团队项目(2011-CXTD-14);; 国家教育部博士点基金项目(20120013130002)

作者(Author): 周俊;张冰;林志健;吴丽丽;朱文静;孙博喻;
ZHOU Jun1,ZHANG Bing1,LIN Zhi-jian1,WU Li-li2,ZHU Wen-jin1,SUN Bo-yu1(1.School of Pharmacology,Beijing University of Traditional Chinese Medicine,Beijing 100102,China; 2.Department of Pharmacy,the Hospital of Ganzhou,Ganzhou 341000,China)

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