中国中药杂志

2018, v.43(14) 2899-2907

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基于形态、显微、多成分测定和指纹图谱的牡丹皮质量评价系统研究
Morphological,microscopic,multiple-component assay and fingerprinting based systematic research on quality evaluation of Moutan Cortex(Paeonia suffruticosa)

王志强;陈勇;刘双双;李洪祥;李卫文;董玲;何春年;陈飞虎;肖培根;
WANG Zhi-qiang;CHEN Yong;LIU Shuang-shuang;LI Hong-xiang;LI Wei-wen;DONG Ling;HE Chun-nian;CHEN Fei-hu;XIAO Pei-gen;School of Pharmacy,Anhui Medical University;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College;Horticultural Research Institute of Anhui Academy of Agricultural Sciences;

摘要(Abstract):

研究拟通过形态、显微、UHPLC多成分测定以及指纹图谱的分析方法联合使用应用于牡丹皮的质量评价。通过牡丹皮根部形态的描述和测量讨论了不同等级牡丹皮成分差异以及其划分依据。牡丹皮粉末和牡丹根部木心粉末的主要显微特征有显著差别,丹皮粉末中含有大量的淀粉粒和草酸钙簇晶,而木心粉末中含有大量的导管。该研究首次报道了在牡丹皮根部横切面木质部中含有清晰的生长年轮,可以用于牡丹生长年限的判断。通过16个成分含量测定比较了牡丹皮主产区安徽亳州和安徽铜陵产牡丹皮药材的差别,发现亳州牡丹皮PGG含量显著高于铜陵牡丹皮(P<0.01)。并联合多成分含量测定和指纹图谱结果比较了不同年限、牡丹皮和木心以及牡丹皮药材和饮片的主要成分含量的差别。作者建议在牡丹皮质量评价标准中增加芍药苷一项,并推荐了含量的最低限量。综上,采用多种方法联合使用的方式可以更系统全面的评价牡丹皮的整体质量差异,为牡丹皮的质量评价方法的优化进行有益的探讨。
The purpose of this study was to combine morphological,microscopic,UHPLC multiple-component assay and fingerprinting studies in order to evaluate the quality of Moutan Cortex(MC) systematically. The root system of Paeonia suffruticosa was measured to compare the morphological variation and the chemical composition of different grades of MC was discussed according to previous studies. The difference between the main microscopic features of MC powder and the xylem powder is dramatic,the MC powder contains great amount of starch granules and clusters of calcium oxalate,while the xylem powder displays considerable vessels. Interestingly,the growth rings of P. suffruticosa was first reported in the xylem of the root transection,this can help to determine the growth years of the plant. Moreover,through the assay of 16 component,MC produced in Tongling and Bozhou in Anhui province were compared,content of PGG in MC produced in Bozhou was significantly higher than MC produced in Tongling(P < 0. 01). MC with different growth years,MC with xylem and unprocessed MC and MC decoction pieces were compared respectively by combining the results of 16 compounds assay and fingerprinting. It is proposed that the quality evaluation standard include the assay of paeoniflorin. Above all,the holistic quality difference can be evaluated more comprehensively by combining multiple analytical methods.

关键词(KeyWords): 牡丹皮;质量评价;形态;生长年轮;UHPLC
Moutan Cortex;quality evaluation;morphological;growth rings;UHPLC

Abstract:

Keywords:

基金项目(Foundation): 国家中医药行业科研专项(201507002);; 国家中药标准化项目(ZYBZH-C-JL-24-03)

作者(Author): 王志强;陈勇;刘双双;李洪祥;李卫文;董玲;何春年;陈飞虎;肖培根;
WANG Zhi-qiang;CHEN Yong;LIU Shuang-shuang;LI Hong-xiang;LI Wei-wen;DONG Ling;HE Chun-nian;CHEN Fei-hu;XIAO Pei-gen;School of Pharmacy,Anhui Medical University;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College;Horticultural Research Institute of Anhui Academy of Agricultural Sciences;

Email:

DOI: 10.19540/j.cnki.cjcmm.20180510.002

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