中国中药杂志

2019, v.44(08) 1537-1544

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近红外光谱法测定染色红花中常见染料的含量
Determination of common dyes in dyed safflower by near infrared spectroscopy

刘攀颜;陈碧清;袁珊珊;杨彬彬;杨婷;石明辉;吕光华;
LIU Pan-yan;CHEN Bi-qing;YUAN Shan-shan;YANG Bin-bin;YANG Ting;SHI Ming-hui;LYU Guang-hua;School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Xinjiang Institute of Chinese Materia Medica and Ethnical Materia Medica;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

由于花冠色红而鲜艳是优质中药红花的主要评价指标,在中药材市场上常有染色红花出现,影响其质量和疗效。为了建立快速测定染色红花的方法,该研究从红花的不同产地和中药材市场上收集了127份红花和染色红花。将近红外光谱(NIRS)与红花的性状鉴别、HPLC、主成分分析、偏最小二乘回归分析相结合,建立NIRS定性、定量检测染色红花及其柠檬黄、胭脂红、日落黄、偶氮玉红、酸性红73及金橙Ⅱ等6种染料含量的方法。结果表明,在NIRS主成分分析图中,50份红花与77份染色红花分布在不同区域,而予以鉴别。在77份染色红花中均检测出柠檬黄(0. 60~3. 66 mg·g-1)、胭脂红(0. 11~1. 37 mg·g-1)及日落黄(0. 10~0. 71 mg·g-1),说明这3种成分是红花的主要染料;而偶氮玉红、酸性红73及金橙Ⅱ未检出。以62份染色红花为校正集样品建立的NIRS测定染料含量的模型,经另外15份染色红花为验证集样品进行预测准确性验证,柠檬黄、胭脂红和日落黄的NIRS预测值与HPLC实测值之间的平均绝对误差(MAD)均小于5%,相关系数分别为0. 970,0. 975,0. 971。说明这2种方法的测定结果一致。由此可见,NIRS不仅可以鉴别红花与染色红花,而且可以测定其染料的含量,用于红花质量的快速检测。
Because the red and bright color of corolla is the main indicator for the quality assessment of good safflower,the dyed safflower is sometimes found at the herbal market,what is influence on this herb quality and efficacy. A total of 127 safflower samples was therefore collected from different cultivating areas and herbal markets in China to develop a rapid method to identify the dyed safflower. Near-infrared spectroscopy(NIRS) combined with characteristic identification,high performance liquid chromatography(HPLC),principal component analysis(PCA) and partial least squares regression analysis(PLS) were employed to differentiate safflower from dyed safflower samples,and further quantify the levels of the 6 dyes,i.e. tartrazine,carmine,sunset yellow,azorubine,acid red 73 and orange Ⅱ in the dyed safflower. The results indicated that the 50 safflower samples and 77 dyed safflower samples were located at different regions in PCA cluster diagram by NIR spectra. Tartrazine,carmineand and sunset yellow were found in the 77 dyed safflower samples with the amounts of 0. 60-3. 66,0. 11-1. 37,0. 10-0. 71 mg·g-1,respectively. It indicated that the three dyes were the common and main dyes in the dyed safflower. However,azorubine,acid red 73 and orange Ⅱ were not detected in all herb samples. A total of 62 dyed safflower samples were chosen as calibration samples to develop the model for estimating the amount of dyes in dyed safflower. The estimating accuracy was verified by another 15 dyed safflower samples. The values of tartrazine,carmine and sunset yellow in dyed safflower samples were compared between the NIRS and HPLC methods. Each value of mean absolute difference(MAD) was less than 5%. The correlation coefficients of tartrazine,carmineand and sunset yellow were 0. 970,0. 975,0. 971,respectively. It indicated the data quantified by NIRS and HPLC were consistence. It is concluded that NIRS can not only differentiate safflower from dyed safflower,but also quantify the amount of the dyes. NIRS is suitable for rapidly identify the quality of safflower.

关键词(KeyWords): 红花;染色;柠檬黄;胭脂红;日落黄;近红外光谱;HPLC
safflower;dyeing;tartrazine;carmine;sunset yellow;near infrared spectroscopy;HPLC

Abstract:

Keywords:

基金项目(Foundation): 四川省教育厅科研创新团队项目(18TD0017);; 成都中医药大学“杏林学者”学科人才科研提升计划项目(CXTD2018018)

作者(Author): 刘攀颜;陈碧清;袁珊珊;杨彬彬;杨婷;石明辉;吕光华;
LIU Pan-yan;CHEN Bi-qing;YUAN Shan-shan;YANG Bin-bin;YANG Ting;SHI Ming-hui;LYU Guang-hua;School of Pharmacy,Chengdu University of Traditional Chinese Medicine;Xinjiang Institute of Chinese Materia Medica and Ethnical Materia Medica;School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20190322.102

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