中国中药杂志

2003, (09)

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苦参注射液生物碱类成分指纹图谱的研究
Study on the Fingerprints of the Alkaloids in Sophora flavescens

马仁玲,周红华,于喜水,盛龙生
MA Ren-ling 1, ZHOU Hong-hua 1, YU Xi-shui 2, SHENG Long-sheng 1 (1.Center for Instrumental Analysis,China pharmaceutical University, Nanji ng 210009, Jiangsu, China; 2. Heilongjiang Academy of Medical Industry,Haerbin 150040, Heilongjiang, C hina)

摘要(Abstract):

目的 :建立中药苦参 (包括药材、中间体及注射液成品 )中生物碱类成分HPLC指纹图谱的测试方法。方法 :利用RP HPLC ,采用线性梯度洗脱等进行指纹图谱的测定 ,并应用LC/MS技术指认指纹图谱中的主要色谱峰。结果 :获得了较为理想的中药苦参生物碱类成分的HPLC指纹图谱 ,比较了苦参药材、中间体及注射液成品间的相关性 ,根据MS所提供的分子量信息 5个色谱峰被指认。结论 :本法可得到精密度、重复性和稳定性较好的中药苦参生物碱类成分的HPLC指纹图谱 ,为中药苦参的质量控制提供有效手段
Objective:To establish a method for HPL C fingerprint determination of the alkaloids in S. flavescens. Method: RP- HPLC,linear grad ient elution,LC/MS,etc. were used to determine the fingerprint and identify th e main pea ks in the HPLC fingerprint. Result: A satisfactory method for HPLC finger print determination of the alkaloids in S. flavescens. was established,and 5 p eaks in the HPLC fingerprint were identified. Conclusion: The perfect fing erprint can be obtained and the method can be used for quality control of S. flavescens.

关键词(KeyWords): 指纹图谱;苦参;生物碱
HPLC fingerprint ;Sophora flavescens ;alkaloids

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作者(Author): 马仁玲,周红华,于喜水,盛龙生
MA Ren-ling 1, ZHOU Hong-hua 1, YU Xi-shui 2, SHENG Long-sheng 1 (1.Center for Instrumental Analysis,China pharmaceutical University, Nanji ng 210009, Jiangsu, China; 2. Heilongjiang Academy of Medical Industry,Haerbin 150040, Heilongjiang, C hina)

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