中国中药杂志

2004, (08)

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药用肉苁蓉的遗传多样性RAPD分析
Study on genetic diversity of Herba Cistanches by RAPD

崔光红,陈敏,黄璐琦,肖苏萍,李达
CUI Guang-hong, CHEN Min, HUANG Lu-qi, XIAO Su-Ping, LI Da(Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China)

摘要(Abstract):

目的 :荒漠肉苁蓉Cistanchedeserticola和管花肉苁蓉Cistanchetubulosa群体遗传多样性的研究。方法 :对荒漠肉苁蓉 2个野生居群和管花肉苁蓉 4个野生居群进行RAPD分析 ,利用POPGENE1.31版软件分析群体的遗传多样性。结果 :荒漠肉苁蓉平均多态位点百分率 4 7.37% ,2个居群多态位点分别为 39.4 7%和 35 .5 3% ,平均Nei’s基因多样性为 0 .135 8,Shannon’s多态性信息指数为 0 .2 0 72 ,基因分化系数Gst=0 .2 5 4 6。管花肉苁蓉平均多态位点百分率 2 7.5 9% ,各居群多态位点百分率从 19.5 4 %到 2 5 .2 9% ,其中安迪尔居群的最高 2 5 .2 9% ,平均Nei’s基因多样性为 0 .0 82 3,Shannon’s多态性信息指数为 0 .12 5 8,基因分化系数Gst=0 .175 5。结论 :荒漠肉苁蓉和管花肉苁蓉在居群间均有一定的分化 ,荒漠肉苁蓉的遗传多样性明显高于管花肉苁蓉 ,并已明显分化成 2个生态型
Objective: To determine the genetic diversity of Cistanche species. Method: Two populations of Cistanche deserticola and four populations of C. tubulosa were analyzed by random amplified polymorphic DNA (RAPD) markers. Result:A total of 76 and 87 loci were amplified using 10 random primers each other. The average percentage of polymorphic loci of C. deserticola was 47.37%. The PPL were 39.47% and 35.53% for two populations. Average Nei's gene diversity was 0.135 8, Shannon's genetic diversity was 0.207 2, and Gst was 0.254 6. The average PPL of C. tubulosa was 27.59%. It was 19.54% to 25.29% in different populations and Andi'er population had the highest. Average Nei's gene diversity was 0.082 3, and Shannon's genetic diversity was 0.125 8, Gst was 0.175 5. Conclusion: The diversity of Cistanche deserticola is higher than that of C. tubulosa, but both has differentiation among populations, C. deserticola has already separated itself into two different ecotypes.

关键词(KeyWords): 荒漠肉苁蓉;管花肉苁蓉;RAPD;遗传多样性
Cistanche deserticola; C. tubulosa; RAPD; genetic diversity

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基金项目(Foundation): 科技部社会公益项目 ( 2 0 0 1.10 9) ;; 科技部“十五”攻关项目 ( 2 0 0 1BA70 1A0 9a)

作者(Author): 崔光红,陈敏,黄璐琦,肖苏萍,李达
CUI Guang-hong, CHEN Min, HUANG Lu-qi, XIAO Su-Ping, LI Da(Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China)

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