中国中药杂志

2000, (06)

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气相色谱-模糊聚类分析法用于红曲基原菌的鉴别
Distinguish of the Original Fungi of the Chinese Traditional Medicine,Hongqu with Gas Chromatography Fuzzy Clustering Analytical Method

邢旺兴,宓鹤鸣,陈斌,陈士景,贺祥,吴玉田
XING Wang xing 1,MI He ming 1,CHEN Bing 1, CHEN Shi jing 2,HE Xiang 1,WU Yu tian 1 (1.School of Pharmacy,Second Military Medical University,Shanghai,200433,China; 2.The 117th Hospital of PLA, Hangzhou 310013,Zhejiang,China) [

摘要(Abstract):

目的 :建立中药红曲基原菌中挥发性成分的气相色谱分析法。方法 :采用毛细管气相色谱法分析橙色红曲霉等 7种红曲霉的挥发性酸和非挥发性酸 ;进样口温度 2 80℃ ,检测器温度 2 80℃ ,程序升温条件 :130℃ ,5min→ 10℃·min-1→ 2 0 0℃ ,10min ,FID检测。结果 :不同种红曲霉挥发性成分、非挥发性成分和含量均存在显著差异 ,根据红曲霉挥发性成分或非挥发性成分指纹图谱的主要峰及其相对含量 ,可以有效地鉴别红曲霉属真菌。结论 :毛细管气相色谱法模糊聚类分析对鉴别红曲霉属真菌具有一定的实用价值。
Objective:To establish a gas chromatography fuzzy clustering analytical method for classifying of origin fungi of the Chinese traditional medicine,Hongqu.Methods:The volatile and nonvolatile components of 7 ordinary species of Monascus including M.aurantiacus Lee,etc. were analyzed by capillary gas chromatog raphy using FID:Inlet temperature 280 ℃,detector temperature 280 ℃,temperature program 130 ℃,5 min→10 ℃·min -1 →200 ℃,10 min.Results:In the peak number and contents of volatile and nonvolatile components of Monascus exist obviously differences through which Monascus can be distinguished easily by the main fingerprint peaks within 20 minutes.Conclusion:The capillary gas chromatography fuzzy clustering analytical method is useful to the identification of Monascus fungi. [

关键词(KeyWords): 红曲;红曲霉菌;气相色谱法;鉴别
Hongqu; Monascus ;gas chromatography;identification

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基金项目(Foundation): 中国人民解放军1995重点课题!(96 Z 0 59);; 国家中医药管理局重点课题!(97 Z 0 97)

作者(Author): 邢旺兴,宓鹤鸣,陈斌,陈士景,贺祥,吴玉田
XING Wang xing 1,MI He ming 1,CHEN Bing 1, CHEN Shi jing 2,HE Xiang 1,WU Yu tian 1 (1.School of Pharmacy,Second Military Medical University,Shanghai,200433,China; 2.The 117th Hospital of PLA, Hangzhou 310013,Zhejiang,China) [

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