中国中药杂志

2010, v.35(21) 2842-2845

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白芥子炒制前后HPLC指纹图谱的比较分析
HPLC fingerprints of processed products from Sinapis alba

张村;李丽;肖永庆;逄镇;李桂柳;
ZHANG Cun,LI Li,XIAO Yongqing,PANG Zhen,LI Guiliu(Institute of Chinese Materia Madica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

摘要(Abstract):

目的:研究建立白芥子不同饮片的指纹图谱测定方法,指认主要色谱峰,分析白芥子炒制前后化学成分的变化情况。方法:采用HPLC,Agilent TC-C18(2)柱,乙腈-0.1%磷酸溶液梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,柱温35℃,以指纹图谱软件计算相似度,并进行图谱比较和指认。结果:各饮片10批次样品平均相似度大于0.96,确定了白芥子生品、炒制品的HPLC标准指纹图谱,并指认了5个主要色谱峰。结论:白芥子炒制前后的HPLC指纹图谱有明显差异,通过2种提取溶剂的比较分析结合色谱峰指认,为揭示白芥子的炒制原理提供了可靠线索。
Objective: To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.Method: The procedure of HPLC analysis was performed on a agilent TC-C18(2)column at 35 ℃ with the acetonitrile-0.1% phosphoric acid in gradient elution as the mobile phase.The detection wavelength was set at 254 nm,and the flow rate was 1.0 mL·min-1.The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(Version 2004 A)was applied to analyze the similarity.Result: The average similarities among the processed products were over 0.96,the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.Conclusion: The established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.

关键词(KeyWords): 白芥子;饮片;HPLC指纹图谱;指认
Sinapis alba;processed products;HPLC fingerprint;identification

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基金项目(Foundation): 国家“十一五”科技支撑计划项目(2006BAI09B06-01);; 国家“重大新药创制”科技重大专项项目(2009ZX09301-005-03)

作者(Author): 张村;李丽;肖永庆;逄镇;李桂柳;
ZHANG Cun,LI Li,XIAO Yongqing,PANG Zhen,LI Guiliu(Institute of Chinese Materia Madica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

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