中国中药杂志

2003, (12)

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葛根药材HPLC指纹图谱研究
Study on HPLC-FPS of Pueraria of Different Sources

何春年,李敏,曹志高,郭红英,王春兰,余世春
HE Chun-nian 1, LI min 2, CAO Zhi-gao 3, GUO Hong-ying 3, WANG Chun-lan 1, YU SHI-chun 2 (1.Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100094, China; 2.Anhui An-tech Medicine&Biological Technology Co., LTD, Hefei 230088, China; 3.Anhui Fang Pharmaceutical Co., LTD, Hefei 230088, China)

摘要(Abstract):

目的 :对不同来源的葛根药材指纹图谱比较研究。方法 :应用RP HPLC法、KromasilC18分析柱、乙腈 水二元梯度洗脱 ,流速为 1.0mL·min-1,检测波长 2 5 0nm。结果与结论 :不同来源的葛根药材所含黄酮类成分均得到很好的分离 ,该法可用于鉴别葛根的品种 ,并为葛根注射液及含葛根药材的中药复方注射液指纹图谱研究奠定基础。
Objective: To make comparative study on HPLC-FPS of several kinds of Pueraria lobata and P. thomsonii from different sources. Method: Kromasil C_ 18 column was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The wavelength of measurement was 250 nm. Result and Conclusion: The fingerprints of P. lobata and P. thomsonii were obtained.This method can be used to identify P. lobata and P. thomsonii from different sources conveniently,and it may be practically valuable for the quality control of sample for P.lobata or P. thomsonii and its preparation.

关键词(KeyWords): 葛根;指纹图谱;高效液相色谱;梯度洗脱
Pueraria lobata; Pueraria thomsonii; HPLC-FPS; gradient elution

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作者(Author): 何春年,李敏,曹志高,郭红英,王春兰,余世春
HE Chun-nian 1, LI min 2, CAO Zhi-gao 3, GUO Hong-ying 3, WANG Chun-lan 1, YU SHI-chun 2 (1.Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100094, China; 2.Anhui An-tech Medicine&Biological Technology Co., LTD, Hefei 230088, China; 3.Anhui Fang Pharmaceutical Co., LTD, Hefei 230088, China)

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