中国中药杂志

2014, v.39(21) 4234-4239

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3种不同基原藏药沙棘的~1H-NMR代谢组学研究
Study on three different species tibetan medicine sea buckthorn by ~1H-NMR-based metabonomics

苏永文;谭尔;张静;游佳莉;刘悦;刘川;周向东;张艺;
SU Yong-wen;TAN Er;ZHANG Jing;YOU Jia-li;LIU yue;LIU Chuan;ZHOU Xiang-dong;ZHANG Yi;College of Ethnomedicine,Chengdu University of Traditional Chinese Medicine;Department of Medicinal Chemistry,College of Pharmacy,Third Military Medical University;

摘要(Abstract):

运用1H-NMR代谢组学方法,建立藏药沙棘的氢核磁共振指纹图谱,明确不同基原之间的整体代谢物差异,为其质量评价提供新方法。利用核磁共振仪测定不同基原的沙棘药材,将所得自由衰减信号导入MestRe Nova软件进行分段积分,数据归一化后进行主成分(PCA)分析和偏最小二乘法(PLS-DA)分析,结果显示3种不同品种沙棘能够明显分开,L-白雀木醇、苹果酸和部分未鉴定的糖类成分是引起3个品种分类的差异代谢物。该研究鉴定出25个代谢产物,包括黄酮类、三萜类、氨基酸类、糖类、脂肪酸类等成分。1H-NMR分析方法具有整体性的特征,并且样品制备简单、分析快速、重复性好,建立的指纹图可以为藏药沙棘药材的质量控制与评价提供参考依据。
The 1H-NMR fingerprints of three different species tibetan medicine sea buckthorn were established by 1H-HMR metabolomics to find out different motablism which could provide a new method for the quality evaluation of sea buckthorn. The obtained free induction decay(FID) signal will be imported into MestRe Nova software and into divide segments. The data will be normalized and processed by principal component analysis and partial least squares discriminant analysis to perform pattern recognition. The results showed that 25 metabolites belonging to different chemical types were detected from sea buckthorn,including flavonoids,triterpenoids,amino acids,carbohydrates,fatty acids,etc. PCA and PLS-DA analysis showed three different varieties of sea buckthorn that can be clearly separated by the content of L-quebrachitol,malic acid and some unidentified sugars,which can be used as the differences metabolites of three species of sea buckthorn.1H-NMR-based metabonomics method had a holistic characteristic with sample preparation and handling. The results of this study can offer an important reference for the species identification and quality control of sea buckthorn.

关键词(KeyWords): 藏药沙棘;1H-NMR代谢组学;化学计量学;质量控制
Tibetan medicine sea buckthorn;1H-NMR metabolomics;chemometrics;quality control

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基金项目(Foundation): 四川省科技支撑计划项目(2013SZ0114);; 四川省教育厅创新团队项目(11TD004);; 四川省教育厅(10ZB075)

作者(Author): 苏永文;谭尔;张静;游佳莉;刘悦;刘川;周向东;张艺;
SU Yong-wen;TAN Er;ZHANG Jing;YOU Jia-li;LIU yue;LIU Chuan;ZHOU Xiang-dong;ZHANG Yi;College of Ethnomedicine,Chengdu University of Traditional Chinese Medicine;Department of Medicinal Chemistry,College of Pharmacy,Third Military Medical University;

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