中国中药杂志

2015, v.40(22) 4442-4445

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多指标成分定量结合指纹图谱分析评价不同来源藏紫草的质量
Multi-component quantitative analysis combined with chromatographic fingerprint for quality assessment of Onosma hookeri

阿呷尔布;聂丽娟;卓玛东智;王聚乐;
AGA Er-bu;NIE Li-juan;DONGZHI Zhuo-ma;WANG Ju-le;Medical College of Tibet University;

摘要(Abstract):

高效液相色谱法-二极管阵列检测器(HPLC-DAD)同时测定藏紫草中紫草素、乙酰紫草素、β,β'-二甲基丙烯酰紫草素的含量,结合藏紫草的HPLC指纹图谱研究,对西藏地区不同来源的藏紫草药材的质量进行初步比较和评价。采用Agilent ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相梯度洗脱,流速0.8 m L·min-1,柱温30℃,检测波长516 nm。利用化学计量法对色谱数据进行相似度比较和聚类分析。测定了7批不同来源藏紫草药材,发现7批药材中3种萘醌类成分的含量差异巨大。指纹图谱相似度比较显示5,6,7号样品间相似度大于0.99,2,3号样品间相似度大于0.97,3,4号样品相似度大于0.90,其余相似度均不超过0.8,7批样品可分为4类,与3种萘醌类成分的含量测定结果基本一致。说明市场上藏紫草药材来源混杂,质量参差不齐。该方法重复性好,可以为不同来源藏紫草药材的质量控制和评价提供依据。
A method for simultaneous determination of the shikonin,acetyl shikonin and β,β'-dimethylpropene shikonin in Onosma hookeri and the chromatographic fingerprint was estabished by HPLC-DAD on an Agilent Zorbax SB-column with a gradient elution of acetonitrile and water at 0. 8 m L·min-1,30 ℃. The quality assessment was conducted by comparing the content difference of three naphthoquinone constituents,in combination with chromatographic fingerprint analysis and systems cluster analysis among 7 batches of radix O. hookeri. The content of the three naphthoquinone constituents showed wide variations in 7 bathces. The similarity value of the fingerprints of sample 5,6 and 7 was above 0. 99,sample 2 and 3 above 0. 97,sample 3 and 4 above 0. 90,and other samples larger than 0. 8,which was in concert with the content of three naphthoquinone constituents. The 7 samples were roughly divided into 4 categories. The results above indicated that the using of this medicine is complex and rather spotty. The established HPLC fingerprints and the quantitative analysis method can be used efficiently for quality assessment of O. hookeri.

关键词(KeyWords): 藏紫草;液相色谱;含量测定;指纹图谱;聚类分析
Onosma hookeri;HPLC;quantitative analysis;fingerprint;systems cluster analysis

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Keywords:

基金项目(Foundation): 西藏自治区基金项目(2012A12G12/00)

作者(Author): 阿呷尔布;聂丽娟;卓玛东智;王聚乐;
AGA Er-bu;NIE Li-juan;DONGZHI Zhuo-ma;WANG Ju-le;Medical College of Tibet University;

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