中国中药杂志

2007, (11) 1060-1064

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天麻糖蛋白的抗凝与抗栓作用
Study of a glycoprotein from Gastrodia elata:its effects of anticoagulation and antithrombosis

丁诚实;沈业寿;李赓;卫自;魏凤;
DING Cheng-shi SHEN Ye-shou LI Geng WEI Zi WEI Feng (Institute of Biochencistry and Nicrobiology,Anhui University,Hefei 230039,China)

摘要(Abstract):

目的:观察天麻糖蛋白(PGE2-1)的抗血凝与抗血栓作用。方法:玻片法、减尾法检测小鼠凝血时间(CT)和出血时间(BT);减尾测5 min后的出血量吸光度(A_(540));小鼠体内测定血浆复钙时间(RT);ADP诱导血小板聚集(PAG);试剂盒测定血浆凝血酶原时间(PT)、凝血酶时间(TT)、部分凝血活酶时间(AFTT);体外血栓仪测定大鼠体外形成血栓的长度、湿重、干重;实验性动静脉旁路血栓模型测定血栓的湿重及PGE2-1对其的抑制率。结果:60,120 mg·kg~(-1)的PGE2-1能显著延长小鼠的凝血时间、出血时间,增大出血量(A_(540))(P<0.05或P<0.01)。30,60,120 mg·kg~(-1)的PGE2-1能延长小鼠血浆复钙时间,降低血小板聚集率(P<0.05或P<0.01)。10, 20,40 mg·mL~(-1)的PGE2-1能使TT和APTT显著延长(P<0.05或P<0.01),但对PT的影响无统计学意义。30, 60,120 mg·kg~(-1)的PGE2-1能使小鼠消除血栓症状、恢复自主活动时间明显缩短(P<0.05或P<0.01);使大鼠体外形成的血栓长度显著减短、湿重和干中显著减小(P<0.05或P<0.01);极显著减小大鼠实验性动脉血栓的湿重(P<0.01),各药物组抑制率分别为32.5%,49.0%和61.5%。结论:PGE2-1具有显著的抗凝、抗栓作用,可能为天麻提取物在抗栓方面的主要成分。
Objective:To investigate the effects of polysaccharide 2-1 from Gastrodia elata(PGE2-1) on blood coagulation and thrombosis.Method:Clotting time(CT)and bleeding time(BT)of mice were measured by glass method and tail-cutting meth- od.Bleeding capacity (A_(540))was measured by cutting tail in 5 min.Plama recalcificatic time(RT)were measured in mice.Platelet ag- gregation was caused by adenosine diphosphate(ADP).Activated partial thromboplastin time(APTT),prothrombin time(PT),and thrombin time(TT)were measured by reagent boxes.During thrombosis in vitro,their lengths,wet and dry weights were measured by instrument;wet weights of arteriovenous experimental thrombosis were measured and the impressive rates were analyzed.Result:CT and BT of groups PGE2-1(60,120 mg·kg~(-1))were remarkably prolonged,and bleeding capacity(A_(540))were significantly increased (P<0.05 or P<0.01).RT of groups PGE2-1(30,60,120 mg·kg~(-1))were remarkably prolonged,and platelet aggregation (PAG)were inhibited(P<0.05 or P<0.01).Human serous TT and APTT of groups PGE2-1(10,20,40 mg·mL~(-1))were re- markably prolonged(P<0.05 or P<0.01),but the difference of effect on PT had no statistic significance.PGE2-1( 30,60,120 mg·kg~(-1))could make the mice obviously eliminate thrombus symptom and reduce the time of restoring independent activity(P<0.05 or P<0.01);thrombosis in vitro:Lengths,wet and dry weights of groups PGE2-1(30,60,120 mg·kg~(-1))were significantly de- creased(P<0.05 or P<0.01);wet weights of arteriovenous experimental thrombosis were dramatically decreased(P<0.01),and impressive rates were respectively 32.5%,49.0% and 61.5%.Conclusion:PGE2-1 has remarkable effects of anticoagulation and antithrombosis,so it may be the main component of the isolation from G.elata in the field of antithrombosis.

关键词(KeyWords): 天麻糖蛋白PGE2-1;抗血凝;抗血栓
PGE2-1;anticoagulation;antithrombosis

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作者(Author): 丁诚实;沈业寿;李赓;卫自;魏凤;
DING Cheng-shi SHEN Ye-shou LI Geng WEI Zi WEI Feng (Institute of Biochencistry and Nicrobiology,Anhui University,Hefei 230039,China)

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